• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human PDGFR beta/CD140b protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

EC50: 1-5 ng/mL

Cat no : Eg0147

Validation Data Gallery

  • Purity of Recombinant Human PDGFR beta was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PDGFR beta was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human PDGFR beta (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human PDGFB (hFc tag) with a linear range of 1-5 ng/mL.

    Immobilized Human PDGFR beta (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human PDGFB (hFc tag) with a linear range of 1-5 ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human PDGFR beta (Myc tag, His tag) at 0.5 μg/mL (100 μL/well) can bind Human PDGFB (hFc tag) with a linear range of 1-5 ng/mL.
Expression HEK293-derived Human PDGFR beta protein Leu33-Lys531 (Accession# P09619-1(S180F)) with a Myc tag and a His tag at the C-terminus.
GeneID 5159
Accession P09619-1
PredictedSize 61.6 kDa
SDS-PAGE 75-110 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Multiple receptor tyrosine kinases and their growth factor ligands have been implicated in cancer progression and metastasis. Among these are the platelet-derived growth factor receptors (PDGFRs). Expression of PDGFRs is mainly restricted to mesenchymal cell types. PDGF is composed of homo-dimers or hetero-dimers of two polypeptide chains, denoted A and B. The two receptor subtypes show different affinities for the dimeric PDGF isoforms. In epithelial tumors, the platelet-derived growth factor receptor B (PDGFRB, also known as PDGFR beta) is mainly expressed by stromal cells of mesenchymal origin. it has also been shown that PDGFRB is also associated with the aggressive behavior of several types of tumors. The 60% of colon cancer patients express high levels of this gene and the PDGFRB expression correlates with lymphatic dissemination of this cancer. Furthermore, PDGFRB signaling in mesenchymal-like tumor cells (as colorectal cancer cells) contributes to invasion and liver metastasis formation.

References:

1.Ernst J A Steller. et al. (2013). Neoplasia. 15(2):204-217.
2.Arne Ostman. et al. (2007). Adv Cancer Res. 97:247-274.
3.Ombretta Melaiu. et al. (2017). Genes Cancer. 8(1-2):438-452.
4.C E Hart. et al. (1988). Science. 240(4858):1529-1531.
5.Thomas C Wehler. et al. (2008). Oncol Rep. 19(3):697-704.