iNOS Recombinant antibody
iNOS Recombinant Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
6O22
Cat no : 80517-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW 264.7 cells, LPS treated human PBMCs |
Positive IHC detected in | mouse brain tissue, rat brain tissue, human liver tissue, human colon tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 1 publications below |
Product Information
80517-1-RR targets iNOS in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Peptide |
Full Name | nitric oxide synthase 2, inducible |
Calculated molecular weight | 131 kDa |
Observed molecular weight | 110-130 kDa |
GenBank accession number | NM_000625 |
Gene symbol | NOS2 |
Gene ID (NCBI) | 4843 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
Protocols
Product Specific Protocols | |
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WB protocol for iNOS antibody 80517-1-RR | Download protocol |
IHC protocol for iNOS antibody 80517-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomed Pharmacother The mechanism of formononetin/calycosin compound optimizing the effects of temozolomide on C6 malignant glioma based on metabolomics and network pharmacology |