iNOS Recombinant antibody

iNOS Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6O22

Cat no : 80517-1-RR

Synonyms

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A



Tested Applications

Positive WB detected inRAW 264.7 cells, LPS treated human PBMCs
Positive IHC detected inmouse brain tissue, rat brain tissue, human liver tissue, human colon tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80517-1-RR targets iNOS in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 2, inducible
Calculated molecular weight 131 kDa
Observed molecular weight110-130 kDa
GenBank accession numberNM_000625
Gene symbol NOS2
Gene ID (NCBI) 4843
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Protocols

Product Specific Protocols
WB protocol for iNOS antibody 80517-1-RRDownload protocol
IHC protocol for iNOS antibody 80517-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols