Tested Applications
| Positive WB detected in | RAW 264.7 cells, LPS treated human PBMCs |
| Positive IHC detected in | mouse brain tissue, human colon tissue, rat brain tissue, human liver tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80517-1-RR targets iNOS in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | nitric oxide synthase 2, inducible |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 110-130 kDa |
| GenBank Accession Number | NM_000625 |
| Gene Symbol | iNOS |
| Gene ID (NCBI) | 4843 |
| RRID | AB_2918898 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35228 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
Publications
What published studies show
Anti-iNOS Rabbit Recombinant Antibody (Cat# 80517-1-RR) has 98 citations across journals including Nature Communications, ACS Nano, Advanced Science, Cell Reports, Redox Biology, International Immunopharmacology, and Journal of Controlled Release. Research fields span macrophage M1/M2 polarization, neuroinflammation, cancer immunotherapy, osteoarthritis, wound healing, spinal cord injury, atherosclerosis, and tissue regeneration, primarily using WB, IF, and IHC in mouse, rat, and human samples.
| Species | Application | Title |
|---|---|---|
Bioact Mater Bioengineered extracellular vesicles escape lysosomal degradation and deliver Tet-PKM2 for macrophage immunometabolic reprogramming and periodontitis treatment. | ||
Nat Commun Inhalable nanovesicles loaded with a STING agonist enhance CAR-T cell activity against solid tumors in the lung | ||
Nat Commun Metabolomics and proteomics reveal blocking argininosuccinate synthetase 1 alleviates colitis in mice | ||
Nat Commun Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury | ||
ACS Nano Biotin-Avidin System-Based Delivery Enhances the Therapeutic Performance of MSC-Derived Exosomes | ||
