ChromoTek iST mNeonGreen-Trap Kit for AP-MS sample preparation of mNeonGreen-fusion proteins

Kit for IP/ Co-IP of mNeonGreen tagged proteins and sample preparation for mass spectrometry (MS)

Specificity

mNeonGreen

Applications

IP, MS

Conjugate

Agarose

Publications

1

Cat no : ntak-iST

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag

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Validation Data Gallery

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  Schematic workflow of iST_mNeonGreen -Tap_kit.

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  Preomics Technology Priciple.

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  Make the most of your time & gain about 40 hours with the iST mNeonGreen-Trap Kit.

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Product Information

Kit for IP/ Co-IP of mNeonGreen tagged proteins and sample preparation for mass spectrometry (MS)

• Applications: IP, MS
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 2 nM
• Specificity/Target: mNeonGreen, Branchiostoma lanceolatum
• Coupled VHH: Recombinant, monoclonal anti-mNeonGreen single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind more than 13 µg of recombinant mNeonGreen
• Elution buffer: Elute the peptides from the cartridge.
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Kit components

Component	Description
mNeonGreen-Trap Agarose	8 reactions plus 2 controls
Digest	Enzyme Trypsin-mix to digest proteins.
Resuspend buffer	Protease reconstitution buffer for enzymes.
Lyse buffer	Denature, reduce and alkylate proteins.
Stop solution	Stop the enzymatic activity.
Wash 1 buffer	Clean up peptides from hydrophobic contaminants.
Wash 2 buffer	Clean up peptides from hydrophilic contaminants.
Elution buffer	Elute the peptides from the cartridge.
LC-Load load	Load peptides on reversed-phase LC-MS column.
Cartridges	Cartridge for 1 to 100 µg protein starting material.
Waste tubes	Tube for collecting waste after washing steps.
Collection tubes	Tube for collecting peptides after elution.
Adapters	Enables placing a cartridge into a tube.
Caps	Cap to optionally close the cartridge’s bottom.

Publications

Application	Title
CoIP	Fungal Genet Biol; Identification and characterisation of sPEPs in Cryptococcus neoformans.; Authors - Paige E Erpf