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Recombinant Mouse IL-2 protein (hFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

EC50: 5-20 ng/mL

Cat no : Eg0456

Validation Data Gallery

  • Purity of Recombinant Mouse IL-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/mL.

    Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 5-20 ng/mL.
Expression HEK293-derived Mouse IL-2 protein Met+Ala21-Gln169 (Accession# P04351) with a human IgG1 Fc tag at the N-terminus.
GeneID 16183
Accession P04351
PredictedSize 43.5 kDa
SDS-PAGE 45-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-2 (Interleukin-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It regulates white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells. IL-2 mediates its effects by binding to IL-2 receptors which are expressed by lymphocytes. IL-2 signal can be transduced by 3 different signaling pathways: JAK/STAT, PI3K/Akt/mTOR and MAPK/ERK pathway. IL-2 plays a role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. It also promotes proliferation of activated B cells and subsequently immunoglobulin production.

References:

1.M C Mingari. et al. (1984). Nature. 312(5995): 641-3.
2.Thomas R Malek. et al. (2010). Immunity. 33(2): 153-65.
3.Wei Liao. et al. (2011). Curr Opin Immunol. 23(5): 598-604.
4.Natalia Arenas Ramirez. et al. (2015). Trends Immunol. 36(12): 763-777.

Publications

SpeciesTitle

Biochem Biophys Res Commun

Immune escape between endoplasmic reticulum stress-related cancer cells and exhausted CD8+T cells leads to neoadjuvant chemotherapy resistance in ovarian cancer

Authors - Siyang Zhang
View Article

Adv Sci (Weinh)

Pyruvate Kinase M2-Responsive Release of Paclitaxel and Indoleamine 2,3-Dioxygenase Inhibitor for Immuno-Chemotherapy of Nonsmall Cell Lung Cancer

Authors - Haisi Wu
View Article