nNOS Polyclonal antibody

nNOS Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 18984-1-AP


bNOS, Constitutive NOS, IHPS1, N NOS, NC NOS, Neuronal NOS, Nitric oxide synthase, brain, nNOS, NOS, NOS type I, NOS1

Tested Applications

Positive IHC detected inmouse heart tissue, mouse kidney tissue, rat testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

18984-1-AP targets nNOS in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nitric oxide synthase 1 (neuronal)
Calculated Molecular Weight 161 kDa
GenBank Accession NumberNM_000620
Gene Symbol NOS1
Gene ID (NCBI) 4842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.


Product Specific Protocols
IHC protocol for nNOS antibody 18984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu

Exp Eye Res

Altered transsulfuration pathway enzymes and redox homeostasis in inherited retinal degenerative diseases.

Authors - Alireza Badiei

Acta Pharmacol Sin

Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion.

Authors - Hu Xia-Min XM

Biochim Biophys Acta Mol Cell Res

NMDA receptor activation induces damage of alveolar type II cells and lung fibrogenesis through ferroptosis

Authors - Hai-Peng Cheng

Eur J Pharmacol

Suppressive effect of nitric oxide synthase (NOS) inhibitor L-NMMA acetate on choroidal fibrosis in experimental myopic guinea pigs through the nitric oxide signaling pathway

Authors - Tuling Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Reyes (Verified Customer) (03-01-2024)

Used nNOS antibody on human cortex brain sections (in red), it worked very nicely

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
nNOS Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:18984-1-AP)