nNOS Polyclonal antibody

nNOS Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18984-1-AP

Synonyms

bNOS, Constitutive NOS, IHPS1, N NOS, NC NOS, Neuronal NOS, Nitric oxide synthase, brain, nNOS, NOS, NOS type I, NOS1

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Validation Data Gallery

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  IHC staining of mouse heart using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse heart tissue, mouse kidney tissue, rat testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

18984-1-AP targets nNOS in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nitric oxide synthase 1 (neuronal)
• Calculated molecular weight: 161 kDa
• GenBank accession number: NM_000620
• Gene symbol: NOS1
• Gene ID (NCBI): 4842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.

Publications

Species	Application	Title
canine	WB,IHC	Exp Eye Res; Altered transsulfuration pathway enzymes and redox homeostasis in inherited retinal degenerative diseases.; Authors - Alireza Badiei
rat	WB	Acta Pharmacol Sin; Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion.; Authors - Hu Xia-Min XM
mouse	WB	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu