nNOS Polyclonal antibody
nNOS Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, ELISA
Conjugate
Unconjugated
Cat no : 18984-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse heart tissue, mouse kidney tissue, rat testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IHC | See 1 publications below |
Product Information
18984-1-AP targets nNOS in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat, canine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | nitric oxide synthase 1 (neuronal) |
Calculated molecular weight | 161 kDa |
GenBank accession number | NM_000620 |
Gene symbol | NOS1 |
Gene ID (NCBI) | 4842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.
Protocols
Product Specific Protocols | |
---|---|
IHC protocol for nNOS antibody 18984-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Exp Eye Res Altered transsulfuration pathway enzymes and redox homeostasis in inherited retinal degenerative diseases. | ||
Acta Pharmacol Sin Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion. | ||
ACS Chem Neurosci Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus. |