|Positive IHC detected in||mouse brain tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18984-1-AP targets nNOS in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||nitric oxide synthase 1 (neuronal)|
|Calculated molecular weight||161 kDa|
|GenBank accession number||NM_000620|
|Gene ID (NCBI)||4842|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
nNOS belongs to the NOS family. NOS1 produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. NOS1 is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of NOS1.
ACS Chem Neurosci
Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.
Acta Pharmacol Sin
Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion.