- Featured Product
- KD/KO Validated
NF-κB p65 Polyclonal antibody
NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10745-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human stomach tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10745-1-AP targets NF-κB p65 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, Bombyx mori, bovine, canine, chicken, fish, hamster, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NF-κB p65 fusion protein Ag1199|
|Full Name||v-rel reticuloendotheliosis viral oncogene homolog A (avian)|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC011603|
|Gene ID (NCBI)||5970|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.
|Product Specific Protocols|
|WB protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|IHC protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|IF protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|IP protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|Click here to view our Standard Protocols|
IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.
REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.
Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy
Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.
Nucleic Acids Res
The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Xie (Verified Customer) (09-29-2022)
NF-κB p65 Antibody staining.
Murali (Verified Customer) (01-24-2022)
Very nice antibody for IHC
Azita (Verified Customer) (05-31-2021)
NSC-34 cells (motor neuron-like cells) strong labelling in WB
Zehua (Verified Customer) (12-17-2019)
After siRNA treatment for 48 hours, specific signal shown here. Works excellent!
Feng-qian (Verified Customer) (12-07-2018)
Fraser (Verified Customer) (06-06-2018)