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NF-κB p65 Polyclonal antibody
NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10745-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, Hek-293 cells, Jurkat cells, MCF-7 cells, K-562 cells, HeLa cells, Raji cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human stomach tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10745-1-AP targets NF-κB p65 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat, CHICKEN, monkey, rabbit, fish, hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NF-κB p65 fusion protein Ag1199|
|Full Name||v-rel reticuloendotheliosis viral oncogene homolog A (avian)|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC011603|
|Gene ID (NCBI)||5970|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.
|Product Specific Protocols|
|WB protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|IHC protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|IF protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|IP protocol for NF-κB p65 antibody 10745-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Maximilian (Verified Customer) (06-30-2023)
Quite a bit of unspecific binding. Did not work in mouse cell lines.
Xie (Verified Customer) (09-29-2022)
NF-κB p65 Antibody staining.
Murali (Verified Customer) (01-24-2022)
Very nice antibody for IHC
Azita (Verified Customer) (05-31-2021)
NSC-34 cells (motor neuron-like cells) strong labelling in WB
Zehua (Verified Customer) (12-17-2019)
After siRNA treatment for 48 hours, specific signal shown here. Works excellent!
Feng-qian (Verified Customer) (12-07-2018)
Fraser (Verified Customer) (06-06-2018)