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  • KD/KO Validated

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (7)





Cat no : 10745-1-AP


NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65

Tested Applications

Positive WB detected inA431 cells, Hek-293 cells, Jurkat cells, MCF-7 cells, K-562 cells, HeLa cells, Raji cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human stomach tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10745-1-AP targets NF-κB p65 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, CHICKEN, monkey, rabbit, fish, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011603
Gene symbol RELA
Gene ID (NCBI) 5970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.


Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IHC protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IF protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IP protocol for NF-κB p65 antibody 10745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




PMID: 32291445

Authors - Kunpeng Wu

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang

Sci Adv

JLP-centrosome is essential for the microtubule-mediated nucleocytoplasmic transport induced by extracellular stimuli.

Authors - Clement M Lee

Nucleic Acids Res

p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway

Authors - Xinxing Zhu

Nat Commun

REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.

Authors - Shuai Wang

Nat Commun

Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy

Authors - Haitao Liu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Maximilian (Verified Customer) (06-30-2023)

Quite a bit of unspecific binding. Did not work in mouse cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mainly Human Breast Cancer Cell lines (MDA-MB-436, MDA-MB-468, MDA-MB-231)

Xie (Verified Customer) (09-29-2022)

NF-κB p65 Antibody staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: hela
NF-κB p65 Antibody Immunofluorescence validation (1:250 dilution) in hela (Cat no:10745-1-AP)

Murali (Verified Customer) (01-24-2022)

Very nice antibody for IHC

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: pharyngeal mucosal
NF-κB p65 Antibody Immunohistochemistry validation (1 to 200 dilution) in pharyngeal mucosal (Cat no:10745-1-AP)

Azita (Verified Customer) (05-31-2021)

NSC-34 cells (motor neuron-like cells) strong labelling in WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Human spinal cord (cervical) tissue

Zehua (Verified Customer) (12-17-2019)

After siRNA treatment for 48 hours, specific signal shown here. Works excellent!

  • Applications: Western Blot, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562
NF-κB p65 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in K562 (Cat no:10745-1-AP)

Feng-qian (Verified Customer) (12-07-2018)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: HaCaT

Fraser (Verified Customer) (06-06-2018)

  • Applications: Other (please state below),