Phospho-TDP43 (Ser409/410) Recombinant antibody

Phospho-TDP43 (Ser409/410) Recombinant Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse and More (1)







Cat no : 80007-1-RR


ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected infrontal cortex of FTLD-TDP type B case, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inCalyculin A treated Jurkat cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80007-1-RR targets Phospho-TDP43 (Ser409/410) in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_007375
Gene Symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.


Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochim Biophys Acta Mol Basis Dis

Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.

Authors - Josephine C Esposto


TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis

Authors - Baggio A Evangelista

Res Sq

Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence

Authors - Joy Mitra

Mol Neurobiol

A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis

Authors - Natasha Vassileff

J Exp Med

A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice

Authors - David Brenner

Mol Cell Neurosci

Cytoplasmic TDP-43 accumulation drives changes in C-bouton number and size in a mouse model of sporadic Amyotrophic Lateral Sclerosis

Authors - Anna Normann Bak


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Silvia (Verified Customer) (08-21-2020)

Excellent phospho-specific TDP-43 antibody. Tested by IHC in ethanol and NBF fixed FTLD-TDP brains and ALS spinal cord. The antibody works well with citrate buffer and FA as antigen retrieval. The dilutions that worked better showing high specificity and low background ranged from 1,000-5,000.Works great also by western blot at dilutions 1:1,000-1:2,000.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000)
  • Cell Tissue Type: human cortex and spinal cord
Phospho-TDP43 (Ser409/410) Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000) dilution) in human cortex and spinal cord (Cat no:80007-1-RR)