Phospho-TDP43 (Ser409/410) Recombinant antibody

Phospho-TDP43 (Ser409/410) Recombinant Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

6M10

Cat no : 80007-1-RR

Synonyms

ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43



Tested Applications

Positive WB detected inEA treatedHeLa cells
Positive IHC detected infrontal cortex of FTLD-TDP type B case, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

80007-1-RR targets Phospho-TDP43 (Ser409/410) in WB, IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name TAR DNA binding protein
Calculated molecular weight 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_007375
Gene symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Protocols

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biochim Biophys Acta Mol Basis Dis

Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.

Authors - Josephine C Esposto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Silvia (Verified Customer) (08-21-2020)

Excellent phospho-specific TDP-43 antibody. Tested by IHC in ethanol and NBF fixed FTLD-TDP brains and ALS spinal cord. The antibody works well with citrate buffer and FA as antigen retrieval. The dilutions that worked better showing high specificity and low background ranged from 1,000-5,000.Works great also by western blot at dilutions 1:1,000-1:2,000.

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence
  • Primary Antibody Dilution: Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000)
  • Cell Tissue Type: human cortex and spinal cord
Phospho-TDP43 (Ser409/410) Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000) dilution) in human cortex and spinal cord (Cat no:80007-1-RR)