• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-12 protein


0.8-4 ng/mL

Specific Activity

minimally 3.00 x 106 IU/mg

Species Reactivity




Cat no : HZ-1256


CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Technical Specifications

GeneID 3592
Species Human
Expression HEK293
Activity(Primary) 0.8-4 ng/mL
Specific Activityminimally 3.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP29459 (p35 subunit) P29460 (p40 subunit)
Molecular Mass 34 and 42 kDa reduced, 57 kDa non-reduced, heterodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).


CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35



Nat Commun

Extracellular mRNA transported to the nucleus exerts translation-independent function.

Authors - Takeshi Tomita

J Mol Cell Cardiol

Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis.

Authors - MakikoOhshima

Front Immunol

Tim-3 Expression Causes NK Cell Dysfunction in Type 2 Diabetes Patients.

Authors - Hui Wang

Immunol Cell Biol

Regulation of human Th9 differentiation by type I interferons and IL-21.

Authors - Michael TWong

J Immunol Methods

In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells.

Authors - LarsBlom

Cell Rep

In vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-γ secretion by natural killer cells

Authors - Xiangyu Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


CHELI (Verified Customer) (01-10-2022)

Performed immune cells stimulation with this recombinant IL-12 and the activation was robust. it worked very well!

  • Applications: Cell culture