Validation Data Gallery
Technical Specifications
| GeneID | 3592,3593 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.8-4 ng/mL |
| Specific Activity | minimally 3.00 x 106 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | P29459 (p35 subunit) P29460 (p40 subunit) |
| Molecular Mass | 34 and 42 kDa reduced, 57 kDa non-reduced, heterodimer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).
Synonyms
CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35
Publications
| Species | Title |
|---|---|
Nat Commun Extracellular mRNA transported to the nucleus exerts translation-independent function. | |
J Mol Cell Cardiol Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis. | |
Immunol Cell Biol Regulation of human Th9 differentiation by type I interferons and IL-21. | |
J Immunol Methods In vitro Th1 and Th2 cell polarization is severely influenced by the initial ratio of naïve and memory CD4+ T cells. | |
Cell Rep In vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-γ secretion by natural killer cells |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH CHELI (Verified Customer) (01-10-2022) | Performed immune cells stimulation with this recombinant IL-12 and the activation was robust. it worked very well!
|
