HumanKine® recombinant human Thrombopoietin protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

Typically ≤ 5 ng/mL EC50

Species Reactivity

human

Purity

>95%

Publications

5

Cat no : HZ-1248

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 7066
• Species: Human
• Expression: HEK293
• Activity: Typically ≤ 5 ng/mL EC50
• Purity: >95%
• Endotoxin: < 1 EU/µg
• Accession Number: P40225
• Molecular Mass: 80 to 85 kDa, monomer, glycosylated.
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Publications

Species	Title
human	ACS Biomater Sci Eng; Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.; Authors - Lidan Chen
human	Sci Rep; A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.; Authors - Dezhi Zhou
human	Oncotarget; Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.; Authors - JingLi
human	J Cell Physiol; HCMV modulates c-Mpl/IEX-1 pathway-mediated megakaryo/thrombopoiesis via PDGFRα and αvβ3 receptors after allo-HSCT.; Authors - Fei-Er Feng
human	J Exp Med; Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.; Authors - AndrewVolk