• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human TPO (Thrombopoietin) protein


100-500 ng/mL EC50 in MO7e cells in defined media

Specific Activity

>1.00 x 106 IU/mg

Species Reactivity




Cat no : HZ-1248


MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Technical Specifications

GeneID 7066
Species Human
Expression HEK293
Activity(Primary) 100-500 ng/mL EC50 in MO7e cells in defined media
Activity(Secondary)1-7 ng/mL EC50 in MO7e cells
Specific Activity>1.00 x 106 IU/mg
Purity >95%
Endotoxin< 1 EU/µg
Accession NumberP40225
Molecular Mass 70 to 93 kDa reduced and non-reduced, monomer, glycosylated.
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


TPO (Thrombopoietin) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).


MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin




PF4 activates the c-Mpl-Jak2 pathway in platelets

Authors - Richard John Buka

J Exp Med

Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.

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J Cell Physiol

HCMV modulates c-Mpl/IEX-1 pathway-mediated megakaryo/thrombopoiesis via PDGFRα and αvβ3 receptors after allo-HSCT.

Authors - Fei-Er Feng

ACS Biomater Sci Eng

Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.

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Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.

Authors - JingLi

Sci Rep

A 3D engineered scaffold for hematopoietic progenitor/stem cell co-culture in vitro.

Authors - Dezhi Zhou