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Animal-free Recombinant Human Thrombopoietin
Humankine®2 Publications

Catalog number: HZ-1248

HEK293 expressed Endotoxin-free Animal-component free

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Freight/Packing £00.00

Format: Lyophilized

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Technical Specifications

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Thrombopoietin activity assay

The activity was determined by the dose- dependent stimulation of the proliferation of MO7e cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

Thrombopoietin purity

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
7066
Species:
Human
Expression:
HEK293
Activity:
Typically ≤ 5 ng/mL EC50
Purity:
>95%
Molecular Mass:
80 to 85 kDa, monomer, glycosylated.
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human TPO is expressed from human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 80 to 85 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media and other applications. C- terminal domain glycosylation is thought to be important for the secretion of TPO from cells and for survival of TPO in the circulation.



Background:

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).



Synonyms:
Thrombopoietin, Megakaryocyte colony-stimulating factor, c-MPL Ligand, MGDF cMPL Ligand, c MPL Ligand,



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