HumanKine® recombinant human TPO (Thrombopoietin) protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

100-500 ng/mL EC50 in MO7e cells in defined media

Specific Activity

>1.00 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1248-GMP

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

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Validation Data Gallery

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  Purity of GMP-grade recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP Recombinant human TPO (HZ-1248-GMP) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of GMP recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 100-500 ng/mL​.

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Technical Specifications

• GeneID: 7066
• Species: Human
• Expression: HEK293
• EC50: 100-500 ng/mL EC50 in MO7e cells in defined media
• Specific Activity: >1.00 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P40225
• Molecular Mass: 70 to 93 kDa reduced and non-reduced, monomer, glycosylated.
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing pH 7.4 0.1% endotoxin-free recombinant human serum albumin (HSA).

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

TPO (Thrombopoietin) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin