CoraLite®594-conjugated vwf Monoclonal antibody

vwf Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F9F3

Cat no : CL594-66682

Synonyms

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-66682

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 vwf antibody (CL594-66682, Clone: 3F9F3 ) at dilution of 1:200.

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  IF Staining of human breast cancer using CL594-66682

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 vwf antibody (CL594-66682, Clone: 3F9F3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66682 targets vwf in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: vwf fusion protein Ag25578
• Full Name: von Willebrand factor
• Gene Symbol: VWF
• Gene ID (NCBI): 7450
• RRID: AB_2920021
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).