VWF Monoclonal antibody

VWF Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IHC, IF

Conjugate

Unconjugated

CloneNo.

3F9F3

Cat no : 66682-1-Ig

Synonyms

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66682-1-Ig targets VWF in IHC, IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VWF fusion protein Ag25578
Full Name von Willebrand factor
Gene symbol VWF
Gene ID (NCBI) 7450
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).

Protocols

Product Specific Protocols
IHC protocol for VWF antibody 66682-1-IgDownload protocol
IF protocol for VWF antibody 66682-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Death Dis

Association of N6-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts.

Authors - Lianpin Wu
mouseIHC

J Cell Mol Med

Impact of chronic intermittent hypoxia on the long non-coding RNA and mRNA expression profiles in myocardial infarction.

Authors - Chaowei Hu