WB results of 22228-1-AP (FOXP3 antibody).

HeLa cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP Result of anti-FOXP3 (IP:22228-1-AP, 3ug; Detection:22228-1-AP 1:1000) with HeLa cells lysate 1840ug.

1x10^6 C57BL/c mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Foxp3 (3G3) (65089-1-Ig, Clone: 3G3) or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21), FITC anti-Mouse IgG1 Antibody, and CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 65089-1-Ig (Foxp3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 BALB/c mouse splenocytes were surface stained with PE Anti-Mouse CD4 (GK1.5) and then fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. Cells were then intracellularly stained with 0.06 ug APC Anti-Mouse Foxp3 (APC-65089, Clone:3G3) or 0.06 ug APC Mouse IgG1 Isotype Control (MOPC-21). It is recommended that the antibody dosage should not exceed 0.06ug.

1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and then fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were then stained with CoraLite® Plus 488-conjugated mouse IgG1 isotype control or 5 ul CoraLite® Plus 488-conjugated Anti-Mouse Foxp3 (CL488-65089, Clone: 3G3).

1X10^6 unstimulated and anti-CD3/CD28-stimulated (with 50 ng/ml TGF-β, 3 days) mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), and then intracellularly stained with 0.06 ug PE Anti-Mouse Foxp3 (PE-65089, Clone:3G3).

1x10^6 mouse splenocytes were intracellularly stained with 0.13 ug CoraLite® Plus 647 Anti-Mouse Foxp3 (3G3) (CL647-65089, Clone:3G3), and 0.13 ug CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5), and 0.13 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

FOXP3 fusion protein Ag17827 SDS-PAGE

FOXP3 fusion protein Ag23085 SDS-PAGE