Tested Applications
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65089-1-Ig targets Foxp3 in IHC, FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | forkhead box P3 |
| GenBank Accession Number | BC132333 |
| Gene Symbol | Foxp3 |
| Gene ID (NCBI) | 20371 |
| RRID | AB_2918392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q99JB6 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Foxp3 antibody 65089-1-Ig | Download protocol |
| IHC protocol for Foxp3 antibody 65089-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse Foxp3 (3G3), catalog number 65089-1-Ig, has 17 citations published in journals including Nature Communications, Advanced Science, Science Advances, Cancer Letters, Journal for ImmunoTherapy of Cancer, Cell Reports, Cell Communications & Signaling, Materials Today Bio, Journal of Translational Medicine, Scientific China Life Sciences, Journal of Ethnopharmacology, Cell Biology International, International Journal of Biochemistry & Cell Biology, Molecular Cell Probes, and Thoracic Cancer. Research fields span tumor immunology, autoimmune diseases, inflammation, and metabolic dysfunction.
| Species | Application | Title |
|---|---|---|
Nat Commun YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer. | ||
Adv Sci (Weinh) Targeting L-Selectin Lymphocytes to Deliver Immunosuppressive Drug in Lymph Nodes for Durable Multiple Sclerosis Treatment | ||
Sci Adv Bcl-xL overexpression in T cells preserves muscle mitochondrial structure and function and prevents frailty in old mice | ||
Cancer Lett BMP9 potentiates immunotherapy in triple-negative breast cancer by suppressing Tregs infiltration via the PRKDC-CCL2 axis. | ||
J Immunother Cancer ADRB2 inhibition suppresses cancer immune evasion by regulating tumor SOX10-PD-L1 axis and T cell function | ||
Cell Commun Signal Tumorous IRE1α facilitates CD8+T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma |
Reviews
What customers say
One verified customer reviewed this product with a 5-star rating in October 2025. The reviewer, Shriya Madan, used the antibody for immunofluorescence and identified it as Anti-Mouse Foxp3 (3G3). No additional comments or detailed feedback were provided beyond the application and product identification.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shriya (Verified Customer) (10-13-2025) | Anti-Mouse Foxp3 (3G3)
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