CoraLite® Plus 647 Anti-Mouse Foxp3 (3G3)

• 

  FC experiment of mouse splenocytes using CL647-65089

  1x10^6 mouse splenocytes were intracellularly stained with 0.125 ug CoraLite® Plus 647 Anti-Mouse Foxp3 (3G3) (CL647-65089, Clone:3G3) or 0.125 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21). Cells were co-stained with CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5) (CL488-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Foxp3 Monoclonal Antibody for FC (Intra)

Cat No. CL647-65089

Clone No.3G3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Mouse

Applications

FC (Intra)

Synonyms

forkhead box P3, Foxp3, JM2, scurfin, sf

---

Tested Applications

• Positive FC (Intra) detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC :

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: forkhead box P3
• GenBank Accession Number: BC132333
• Gene Symbol: Foxp3
• Gene ID (NCBI): 20371
• RRID: AB_2920252
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).