FOXP3 Polyclonal antibody

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  WB analysis of HEK-293 using 22228-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 22228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 22228-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22228-1-AP (FOXP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 22228-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using FOXP3 antibody (22228-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CD3 antibody (60181-1-Ig, Clone: 3F3A1, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FOXP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 22228-1-AP

Publications(82)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, Forkhead box protein P3 41 kDa form

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Tested Applications

• Positive WB detected in: HEK-293 cells, A375 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 51 publications below
• IHC: See 24 publications below
• IF: See 12 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

22228-1-AP targets FOXP3 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXP3 fusion protein Ag17571
• Full Name: forkhead box P3
• Calculated Molecular Weight: 431 aa, 47 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC113401
• Gene Symbol: FOXP3
• Gene ID (NCBI): 50943
• RRID: AB_11182376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZS1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publications

Species	Application	Title
mouse	IF	Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
mouse	IF	Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
mouse	IHC	Biomaterials; LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.; Authors - Feng Liu
human	WB	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
human	WB	J Autoimmun; NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.; Authors - Mengxiao Xie
human	WB	Biomed Pharmacother; Glycyrrhetinic acid inhibits non-small cell lung cancer via promotion of Prdx6- and caspase-3-mediated mitochondrial apoptosis; Authors - Qiuyan Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: brain slices from mouse