FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22228-1-AP

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID



Tested Applications

Positive WB detected inHeLa cells, multi-cells, multi-cells/tissue, A375 cells, mouse brain tissue, HEK-293 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22228-1-AP is FOXP3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXP3 fusion protein Ag17571
Full Name forkhead box P3
Calculated molecular weight 431 aa, 47 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC113401
Gene symbol FOXP3
Gene ID (NCBI) 50943
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Protocols

Product Specific Protocols
WB protocol for FOXP3 antibody 22228-1-APDownload protocol
IP protocol for FOXP3 antibody 22228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Biomaterials

LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.

Authors - Feng Liu
humanWB

Clin Transl Med

MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.

Authors - Yuanyuan Chang
humanWB

J Autoimmun

NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.

Authors - Mengxiao Xie
humanIHC

Cancer Lett

A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.

Authors - Huimin Tao
humanIHC

Thyroid

Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.

Authors - Kai Guo
humanIHC

Front Immunol

Bifidobacterium infantis Promotes Foxp3 Expression in Colon Cells via PD-L1-Mediated Inhibition of the PI3K-Akt-mTOR Signaling Pathway.

Authors - Linyan Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: brain slices from mouse