FOXP3 Polyclonal antibody

FOXP3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

53

Reviews (1)

Cat no : 22228-1-AP

Synonyms

AIID, DIETER, forkhead box P3, Forkhead box protein P3, FOXP3, IPEX, JM2, PIDX, Scurfin, XPID

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Validation Data Gallery

• 

  WB analysis of multi-cells/tissue using 22228-1-AP

  WB results of 22228-1-AP (FOXP3 antibody).

• 

  WB analysis of HeLa using 22228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 22228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 22228-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22228-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22228-1-AP (FOXP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 22228-1-AP

  WB results of anti-22228-1-AP (FOXP3 antibody).

• 

  IP experiment of HeLa using 22228-1-AP

  IP Result of anti-FOXP3 (IP:22228-1-AP, 3ug; Detection:22228-1-AP 1:1000) with HeLa cells lysate 1840ug.

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Tested Applications

• Positive WB detected in: HeLa cells, multi-cells, multi-cells/tissue, A375 cells, mouse brain tissue, HEK-293 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 34 publications below
• IHC: See 18 publications below
• IF: See 4 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 22228-1-AP is FOXP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXP3 fusion protein Ag17571
• Full Name: forkhead box P3
• Calculated molecular weight: 431 aa, 47 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC113401
• Gene symbol: FOXP3
• Gene ID (NCBI): 50943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXP3, also named as IPEX, JM2 and Scurfin, is a transcription factor. POXP3 plays a critical role in the control of immune response. Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human FOXP3.

Publications

Species	Application	Title
mouse	IF	Acta Pharm Sin B; Self-assembled FeS-based cascade bioreactor with enhanced tumor penetration and synergistic treatments to trigger robust cancer immunotherapy; Authors - Hao Ren
mouse	IF	Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
mouse	IHC	Biomaterials; LncRNA-targeting bio-scaffold mediates triple immune effects for postoperative colorectal cancer immunotherapy.; Authors - Feng Liu
human	WB	Clin Transl Med; MiRNA-516a promotes bladder cancer metastasis by inhibiting MMP9 protein degradation via the AKT/FOXO3A/SMURF1 axis.; Authors - Yuanyuan Chang
human	WB	J Autoimmun; NF-κB-driven miR-34a impairs Treg/Th17 balance via targeting Foxp3.; Authors - Mengxiao Xie
rat	WB	Biomed Pharmacother; Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.; Authors - Yu Long

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anne-Laure (Verified Customer) (12-07-2021)

non-specific staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: brain slices from mouse

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