PE Anti-Mouse Foxp3 (3G3)

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  FC experiment of mouse splenocytes using PE-65089

  1x10^6 mouse splenocytes were intracellularly stained with 0.06 ug PE Anti-Mouse Foxp3 (3G3) (PE-65089, Clone: 3G3) or 0.06 ug PE Mouse IgG1 Isotype Control (MOPC-21) (PE-65124, Clone: MOPC-21). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Foxp3 Monoclonal Antibody for FC (Intra)

Cat No. PE-65089

Clone No.3G3

Publications(10)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

mouse and More (1)

Applications

FC (Intra)

Synonyms

3G3, forkhead box P3, Forkhead box protein P3 41 kDa form, Forkhead box protein P3, C-terminally processed, JM2

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Tested Applications

• Positive FC (Intra) detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 6 publications below

Product Information

PE-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: forkhead box P3
• GenBank Accession Number: BC132333
• Gene Symbol: Foxp3
• Gene ID (NCBI): 20371
• RRID: AB_2883883
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q99JB6
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	FC (Intra)	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
mouse	FC	Cancer Lett; BMP9 potentiates immunotherapy in triple-negative breast cancer by suppressing Tregs infiltration via the PRKDC-CCL2 axis.; Authors - Yiqing You
mouse	FC	Adv Sci (Weinh); Multifunctional Nano Immunostimulant: Overcoming Immunosuppressive Microenvironment for Antitumor Immunotherapy.; Authors - Guanhong Guo
rat	FC	Ann Med; Chlorogenic acid mitigates acute respiratory distress syndrome via inhibition of the PI3K/AKT signalling pathway: an integrated analysis of bioinformatics and validation experiments.; Authors - Jie Wei
mouse	FC	J Adv Res; Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis; Authors - Yi Chen
mouse	FC	Nat Aging; DNA damage in macrophages drives immune autoreactivity via nuclear antigen presentation.; Authors - George Niotis