Tested Applications
| Positive FC (Intra) detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | forkhead box P3 |
| GenBank Accession Number | BC132333 |
| Gene Symbol | Foxp3 |
| Gene ID (NCBI) | 20371 |
| RRID | AB_2883883 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q99JB6 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE Foxp3 antibody PE-65089 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-65089 is Anti-Mouse Foxp3 (3G3), a monoclonal antibody with 10 citations. These appear in journals including Nature Aging, Cell Reports, Journal of Advanced Research, Advanced Science, Cancer Letters, Phytomedicine, Journal of Colloid and Interface Science, Annals of Medicine, Genes & Immunity, and Human Cell. The research fields span tumor immunotherapy, autoimmune diseases, lupus nephritis, acute respiratory distress syndrome, periodontitis, and graft-versus-host disease.
| Species | Application | Title |
|---|---|---|
Nat Aging DNA damage in macrophages drives immune autoreactivity via nuclear antigen presentation. | ||
J Adv Res Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis | ||
Adv Sci (Weinh) Multifunctional Nano Immunostimulant: Overcoming Immunosuppressive Microenvironment for Antitumor Immunotherapy. | ||
Cancer Lett BMP9 potentiates immunotherapy in triple-negative breast cancer by suppressing Tregs infiltration via the PRKDC-CCL2 axis. | ||
Phytomedicine Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma. | ||
J Colloid Interface Sci Nano co-inducer of immunogenic cell death and ferroptosis for anti-tumor immunotherapy |
