PE Anti-Mouse Foxp3 (3G3)

Foxp3 Monoclonal Antibody for FC (Intra)

Cat No. PE-65089
Clone No.3G3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

mouse

Applications

FC (Intra)

sf, scurfin, JM2, forkhead box P3, 3G3

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC (Intra) detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

PE-65089 targets Foxp3 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A Predict reactive species
Full Name forkhead box P3
GenBank Accession NumberBC132333
Gene Symbol Foxp3
Gene ID (NCBI) 20371
RRIDAB_2883883
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
FormLiquid
Purification MethodAffinity purification
UNIPROT IDQ99JB6
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Protocols

Product Specific Protocols
FC protocol for PE Foxp3 antibody PE-65089Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC (Intra)

Phytomedicine

Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.

Authors - Li Wu
mouseFC (Intra)

Hum Cell

Tetramerization of pyruvate kinase M2 attenuates graft-versus-host disease by inhibition of Th1 and Th17 differentiation

Authors - Meng Wang

Cell Rep

Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses

Authors - Bin Qian
mouseFC

J Adv Res

Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis

Authors - Yi Chen
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