APC Anti-Mouse Foxp3 (3G3)

Foxp3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

3G3

Cat no : APC-65089

Synonyms

forkhead box P3, Foxp3, JM2, scurfin, sf



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

APC-65089 targets Foxp3 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name forkhead box P3
GenBank accession numberBC132333
Gene symbol Foxp3
Gene ID (NCBI) 20371
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/660 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

Foxp3 (forkhead box P3), also named as IPEX, JM2 and scurfin, is a member of the forkhead transcription factor family and is essential for normal immune homeostasis (PMID: 11138001; 20429413). Foxp3 is specifically expressed in CD4+ CD25+ regulatory T cells and is crucial for their development and suppressive activity (PMID: 12612578; 12612581). In scurfy mouse, disruption of Foxp3 results in the fatal lymphoproliferative disorder attributed to the lack of an immune-suppressive component in the immune system (PMID: 25683611; 11138001). Similar to mice, genetic defects in human Foxp3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) which also known as X-linked autoimmunity-immunodeficiency syndrome (PMID: 11137993).

Protocols

Product Specific Protocols
FC protocol for APC Foxp3 antibody APC-65089Download protocol
Standard Protocols
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