A375 cells were subjected to SDS PAGE followed by western blot with 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP Result of anti-14-3-3 epsilon (IP:11648-2-AP, 3ug; Detection:11648-2-AP 1:1000) with A375 cells lysate 6000ug.

Immunofluorescent analysis of HepG2 cells, using YWHAE antibody 11648-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11648-2-AP (14-3-3 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HepG2 cells were stained with 0.2ug 14-3-3 epsilon antibody (11648-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Various lysates were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-14-3-3 (IP:14503-1-AP, 5ug; Detection:14503-1-AP 1:500) with mouse lung tissue lysate 4000ug.

Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (14503-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Various lysates were subjected to SDS PAGE followed by western blot with 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

siRNA result of 12381-1-AP (anti-14-3-3G) in Mouse IMCD3 cells From Dr.Seongjin Seo.

IP result of anti-14-3-3 GAMMA-Specific (IP:12381-1-AP, 3ug; Detection:12381-1-AP 1:800) with mouse brain tissue lysate 8000ug.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12381-1-AP (14-3-3 GAMMA-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of MDCK cells using 12381-1-AP (14-3-3 GAMMA-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IP result of anti-14-3-3(IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (66061-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens).

Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human 14-3-3 Sigma (66251-1-Ig, Clone:3F10D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 10x lens).

1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human 14-3-3 Sigma (10622-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66061 (14-3-3 antibody) at dilution of 1:50.

Various lysates were subjected to SDS PAGE followed by western blot with 66061-1-Ig (14-3-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

IP result of anti-14-3-3 (IP:66061-1-Ig, 4ug; Detection:66061-1-Ig 1:20000) with rat brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66061-1-Ig (14-3-3 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of HepG2 cells using 66061-1-Ig (14-3-3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (66061-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500. This data was developed using the same antibody clone with 66061-1-PBS in a different storage buffer formulation.