14-3-3 Sigma Monoclonal antibody

14-3-3 Sigma Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3F10D9

Cat no : 66251-1-Ig

Synonyms

14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS



Tested Applications

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:8000
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66251-1-Ig targets 14-3-3 Sigma in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 14-3-3 Sigma fusion protein Ag12644
Full Name stratifin
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC002995
Gene Symbol SFN
Gene ID (NCBI) 2810
RRIDAB_2881639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
IHC protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
IF protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
FC protocol for 14-3-3 Sigma antibody 66251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Cell Biol

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Authors - Yoshihiko Kobayashi
humanWB,IHC,IF

Mol Biotechnol

Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling

Authors - Naiyi Du
humanIHC

Transl Androl Urol

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Authors - Xuechao Li
humanIHC

Environ Toxicol

Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer

Authors - Hua Chen