14-3-3 Sigma Polyclonal antibody

14-3-3 Sigma Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10622-1-AP

Synonyms

14 3 3 protein sigma, 14-3-3, 14-3-3 sigma, HME1, SFN, stratifin, YWHAS



Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inmouse skin tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

10622-1-AP targets 14-3-3 Sigma in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 Sigma fusion protein Ag0973
Full Name stratifin
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC002995
Gene symbol SFN
Gene ID (NCBI) 2810
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 Sigma antibody 10622-1-APDownload protocol
IHC protocol for 14-3-3 Sigma antibody 10622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols