14-3-3 Monoclonal antibody

14-3-3 Monoclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, rat, mouse, pig







Cat no : 66061-1-Ig


14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 14-3-3 tau, HS1, Protein HS1, YWHAQ

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman lung cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66061-1-Ig targets 14-3-3 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 14-3-3 fusion protein Ag6055
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 31 kDa, 28 kDa
GenBank Accession NumberBC056867
Gene Symbol YWHAQ
Gene ID (NCBI) 10971
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

14-3-3 proteins interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.


Product Specific Protocols
WB protocol for 14-3-3 antibody 66061-1-IgDownload protocol
IHC protocol for 14-3-3 antibody 66061-1-IgDownload protocol
IF protocol for 14-3-3 antibody 66061-1-IgDownload protocol
IP protocol for 14-3-3 antibody 66061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Aging (Albany NY)

In vitro and in vivo efficacy of the novel oral proteasome inhibitor NNU546 in multiple myeloma.

Authors - Hui Zhou

Front Immunol

Interactome of the Autoimmune Risk Protein ANKRD55.

Authors - Nerea Ugidos

Neurosci Res

HAP1 interacts with 14-3-3 to regulate epileptic seizure via GABAAR-mediated inhibitory synaptic transmission in pentylenetetrazole rat model.

Authors - Yuetao Wen

Clin Nutr

Ceramide enhanced the hepatic glucagon response through regulation of CREB activity

Authors - Jizheng Wang