- Featured Product
- KD/KO Validated
14-3-3 Epsilon Polyclonal antibody
14-3-3 Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, FC, ELISA
Cat no : 11648-2-AP
Validation Data Gallery
|Positive WB detected in||A375 cells, HeLa cells|
|Positive IP detected in||A375 cells|
|Positive IHC detected in||human lung cancer tissue, human gliomas tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 10 publications below|
|IF||See 4 publications below|
|IP||See 2 publications below|
11648-2-AP targets 14-3-3 Epsilon in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||14-3-3 Epsilon fusion protein Ag2247|
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|
|Calculated molecular weight||255 aa, 29 kDa|
|Observed molecular weight||29-32 kDa|
|GenBank accession number||BC000179|
|Gene ID (NCBI)||7531|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
14-3-3 Epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against full-length 14-3-3 Epsilon.
|Product Specific Protocols|
|WB protocol for 14-3-3 Epsilon antibody 11648-2-AP||Download protocol|
|IHC protocol for 14-3-3 Epsilon antibody 11648-2-AP||Download protocol|
|IF protocol for 14-3-3 Epsilon antibody 11648-2-AP||Download protocol|
|IP protocol for 14-3-3 Epsilon antibody 11648-2-AP||Download protocol|
|FC protocol for 14-3-3 Epsilon antibody 11648-2-AP||Download protocol|
|Click here to view our Standard Protocols|
A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.
Cell Host Microbe
Zika Virus NS3 Mimics a Cellular 14-3-3-Binding Motif to Antagonize RIG-I- and MDA5-Mediated Innate Immunity.
AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.
Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.
1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.
Interactome of the Autoimmune Risk Protein ANKRD55.