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  • KD/KO Validated

14-3-3 Polyclonal antibody

14-3-3 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14503-1-AP


14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse brain tissue, Jurkat cells, rat liver tissue, mouse heart tissue, NIH/3T3 cells, C6 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14503-1-AP targets 14-3-3 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 fusion protein Ag5959
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC056867
Gene Symbol YWHAQ
Gene ID (NCBI) 10971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.


Product Specific Protocols
WB protocol for 14-3-3 antibody 14503-1-APDownload protocol
IHC protocol for 14-3-3 antibody 14503-1-APDownload protocol
IF protocol for 14-3-3 antibody 14503-1-APDownload protocol
IP protocol for 14-3-3 antibody 14503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Targeting 14-3-3θ-mediated TDP-43 pathology in amyotrophic lateral sclerosis and frontotemporal dementia mice

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APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

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Aging (Albany NY)

The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.

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J Inflamm Res

PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.

Authors - Bo Huang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great for western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Renal Cancer cell line