14-3-3 Polyclonal antibody

• Featured Product
• KD/KO Validated

14-3-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 14503-1-AP

Synonyms

14 3 3, 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14-3-3, 1C5, HS1, Protein HS1, YWHAQ

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Validation Data Gallery

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  WB analysis using 14503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14503-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14503-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14503-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14503-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 14503-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14503-1-AP (14-3-3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 14503-1-AP

  IP Result of anti-14-3-3 (IP:14503-1-AP, 5ug; Detection:14503-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of mouse brain using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14503-1-AP (14-3-3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14503-1-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 14503-1-AP

  Immunofluorescent analysis of HepG2 cells, using YWHAQ antibody 14503-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14503-1-AP

  1X10^6 HeLa cells were stained with 0.2ug 14-3-3 antibody (14503-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, rat liver tissue, mouse liver tissue, mouse brain tissue, mouse heart tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

14503-1-AP targets 14-3-3 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: 14-3-3 fusion protein Ag5959
• Full Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• Calculated molecular weight: 28 kDa
• Observed molecular weight: 31 kDa
• GenBank accession number: BC056867
• Gene symbol: YWHAQ
• Gene ID (NCBI): 10971
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

Publications

Species	Application	Title
human	WB	Elife; Ser/Thr kinase Trc controls neurite outgrowth in Drosophila by modulating microtubule-microtubule sliding.; Authors - Rosalind Norkett ; KD Validated
human,mouse	WB,IP	Aging (Albany NY); The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.; Authors - Chunyan Li
human	WB	J Inflamm Res; PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles.; Authors - Bo Huang
rat	IP	Biochim Biophys Acta Mol Basis Dis; Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination.; Authors - Xiaoliang Liu
human	WB,IP	J Biol Chem; 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation; Authors - Anirban Kundu ; KD Validated
	WB	Commun Biol; HMGB1 signaling phosphorylates Ku70 and impairs DNA damage repair in Alzheimer's disease pathology.; Authors - Hikari Tanaka

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for western blot and it is working great for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Renal Cancer cell line