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14-3-3 GAMMA-Specific Polyclonal antibody

14-3-3 GAMMA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, dog

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12381-1-AP

Synonyms

14 3 3 protein gamma, 14 3 3GAMMA, 14-3-3, 14-3-3 GAMMA, KCIP 1, YWHAG



Tested Applications

Positive WB detected inmouse IMCD3 cells (RNAi), A431 cells, K-562 cells, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12381-1-AP targets 14-3-3 GAMMA-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, dog samples.

Tested Reactivity human, mouse, rat, dog
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen 14-3-3 GAMMA-Specific fusion protein Ag3047
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 28-33 kDa
GenBank accession numberBC020963
Gene symbol YWHAG
Gene ID (NCBI) 7532
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.

Protocols

Product Specific Protocols
WB protocol for 14-3-3 GAMMA-Specific antibody 12381-1-APDownload protocol
IHC protocol for 14-3-3 GAMMA-Specific antibody 12381-1-APDownload protocol
IF protocol for 14-3-3 GAMMA-Specific antibody 12381-1-APDownload protocol
IP protocol for 14-3-3 GAMMA-Specific antibody 12381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Exp Clin Cancer Res

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Authors - Jia Song
humanCoIP

Autophagy

YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization.

Authors - Yang Xu
humanWB

Proteomics

Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.

Authors - Songlin Zeng