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14-3-3 GAMMA-Specific Polyclonal antibody
14-3-3 GAMMA-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 12381-1-AP
Validation Data Gallery
|Positive WB detected in||mouse IMCD3 cells (RNAi), A431 cells, K-562 cells, NIH/3T3 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12381-1-AP targets 14-3-3 GAMMA-Specific in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, dog samples.
|Tested Reactivity||human, mouse, rat, dog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||14-3-3 GAMMA-Specific fusion protein Ag3047|
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide|
|Calculated molecular weight||247 aa, 28 kDa|
|Observed molecular weight||28-33 kDa|
|GenBank accession number||BC020963|
|Gene ID (NCBI)||7532|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.
|Product Specific Protocols|
|WB protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP||Download protocol|
|IHC protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP||Download protocol|
|IF protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP||Download protocol|
|IP protocol for 14-3-3 GAMMA-Specific antibody 12381-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Clin Cancer Res
14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.
YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization.
Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected vero cells.