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  • KD/KO Validated

ACAD9 Polyclonal antibody

ACAD9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15770-1-AP

Synonyms

ACAD 9, ACAD9



Tested Applications

Positive WB detected inHEK-293T cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15770-1-AP targets ACAD9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAD9 fusion protein Ag8414
Full Name acyl-Coenzyme A dehydrogenase family, member 9
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC007970
Gene Symbol ACAD9
Gene ID (NCBI) 28976
RRIDAB_2241997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency).

Protocols

Product Specific Protocols
WB protocol for ACAD9 antibody 15770-1-APDownload protocol
IHC protocol for ACAD9 antibody 15770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

Authors - Cong Wang
WB

Sci Rep

Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.

Authors - David W Reid
humanWB

Cell Rep

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Authors - Hezhi Fang
  • KD Validated
WB

Front Neurosci

Ultrasound stimulation of the vagal nerve improves acute septic encephalopathy in mice

Authors - Yukio Imamura