ACAD9 Polyclonal antibody

ACAD9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15770-1-AP

Synonyms

ACAD 9, ACAD9



Tested Applications

Positive WB detected inHEK-293T cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

15770-1-AP targets ACAD9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAD9 fusion protein Ag8414
Full Name acyl-Coenzyme A dehydrogenase family, member 9
Calculated molecular weight 69 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC007970
Gene symbol ACAD9
Gene ID (NCBI) 28976
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency).

Protocols

Product Specific Protocols
WB protocol for ACAD9 antibody 15770-1-APDownload protocol
IHC protocol for ACAD9 antibody 15770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs.

Authors - David W Reid
humanWB

EMBO Rep

MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.

Authors - Cong Wang