ADCY6 Polyclonal antibody

ADCY6 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14616-1-AP

Synonyms

AC6, ADCY6, adenylate cyclase 6, Adenylate cyclase type 6, Adenylate cyclase type VI, Adenylyl cyclase 6, ATP pyrophosphate lyase 6, KIAA0422



Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

14616-1-AP targets ADCY6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADCY6 fusion protein Ag6218
Full Name adenylate cyclase 6
Calculated Molecular Weight 131 kDa
Observed Molecular Weight 131 kDa
GenBank Accession NumberBC064923
Gene Symbol ADCY6
Gene ID (NCBI) 112
RRIDAB_2242343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADCY6 antibody 14616-1-APDownload protocol
IP protocol for ADCY6 antibody 14616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Authors - Xinzhi Li
mouseWB

Mol Metab

Purβ promotes hepatic glucose production by increasing Adcy6 transcription.

Authors - Linna Jia
humanWB

Mol Pharmacol

Activity of adenylyl cyclase type 6 is suppressed by direct binding of the cytoskeletal protein 4.1G.

Authors - Masaki Saito
humanWB

Pathol Oncol Res

Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway.

Authors - Ye Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Charlotte (Verified Customer) (07-27-2022)

1 and 2 : WT starved and ShhN stimulated. 3 to 6 are ADC6 KO cells with 2 different gRNAs. 3/4 is one gRNA and 5/6 is another one. 3 and 5 are starved while 4 and 6 are ShhN stimulated

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: NIH-3T3
ADCY6 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:14616-1-AP)