Tested Applications
| Positive WB detected in | HT-29 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue |
| Positive IHC detected in | human colon tissue, human colon cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6382 Product name: Recombinant human ADHFE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC064634 Sequence: MAVSNIRYGAAVTKEVGMDLKNMGAKNVCLMTDKNLSKLPPVQVAMDSLVKNGIPFTVYDNVRVEPTDSSFMEAIEFAQKGAFDAYVAVGGGSTMDTCKAANLYASSPHSDFLDYVSAPIGKGKPVSVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKIGITSRAIKPTLGLIDPLHTLHMPARVVANSGFDVLCHALESYTTLPYHLRSPCPSNPITRPAYQGSNPISDIWAIHALRIVAKYLKRAVNSTDK Predict reactive species |
| Full Name | alcohol dehydrogenase, iron containing, 1 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50 kDa, 40-45 kDa |
| GenBank Accession Number | BC064634 |
| Gene Symbol | ADHFE1 |
| Gene ID (NCBI) | 137872 |
| RRID | AB_2221451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWW8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADHFE1 antibody 14674-1-AP | Download protocol |
| WB protocol for ADHFE1 antibody 14674-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADHFE1 Polyclonal antibody (Cat# 14674-1-AP) has 4 total citations. These appear in Cancer Letters, Cell Chemical Biology, Circulation Cardiovascular Genetics, and FASEB Journal. The associated research fields span lung cancer immune evasion, biosensor development, diabetic vascular disease, and cardiac hypertrophy involving ferroptosis regulation.
| Species | Application | Title |
|---|---|---|
Cancer Lett HHLA2 promotes immune evasion in EGFR-mutant lung cancer by inhibiting CD8(+) T cell glutamine metabolism via KIR3DL3 interaction. | ||
Cell Chem Biol Development of D2HG biosensors inspired by the molecular mechanism of D2HG regulation of DhdR. | ||
Circ Cardiovasc Genet Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease. | ||
FASEB J Modulation of Ferroptosis by the Irx3-Etfa Pathway Protects Against Cardiac Hypertrophy. |
