ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19359-1-AP

Synonyms

ADH8, ADHFE1, HMFT2263, HOT



Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19359-1-AP is ADHFE1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADHFE1 fusion protein Ag6803
Full Name alcohol dehydrogenase, iron containing, 1
Calculated molecular weight 50 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC064634
Gene symbol ADHFE1
Gene ID (NCBI) 137872
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADHFE1 antibody 19359-1-APDownload protocol
IHC protocol for ADHFE1 antibody 19359-1-APDownload protocol
IP protocol for ADHFE1 antibody 19359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols