ADRP/Perilipin 2 Monoclonal antibody

ADRP/Perilipin 2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5D4

Cat no : 60340-1-Ig

Synonyms

ADFP, Adipophilin, ADRP, Perilipin 2, PLIN2



Tested Applications

Positive WB detected inhuman adipose tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60340-1-Ig targets ADRP/Perilipin 2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADRP/Perilipin 2 fusion protein Ag7643
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC005127
Gene symbol ADFP
Gene ID (NCBI) 123
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
WB protocol for ADRP/Perilipin 2 antibody 60340-1-IgDownload protocol
IHC protocol for ADRP/Perilipin 2 antibody 60340-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols