Angiotensinogen Polyclonal antibody

Angiotensinogen Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 11992-1-AP

Synonyms

AGT, Ang I Angiotensin 2, Ang II Angiotensin 3, Ang III, Angiotensin, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensinogen, ANHU, Des Asp[1] angiotensin II, Serpin A8, SERPINA8



Tested Applications

Positive WB detected inHuH-7 cells, HepG2 cells, human plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11992-1-AP targets Angiotensinogen in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Angiotensinogen fusion protein Ag2632
Full Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Calculated molecular weight 485 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC011519
Gene symbol AGT
Gene ID (NCBI) 183
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Protocols

Product Specific Protocols
WB protocol for Angiotensinogen antibody 11992-1-APDownload protocol
IHC protocol for Angiotensinogen antibody 11992-1-APDownload protocol
FC protocol for Angiotensinogen antibody 11992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Small

Highly Porous Microcarriers for Minimally Invasive In Situ Skeletal Muscle Cell Delivery.

Authors - Ranjith Kumar Kankala
mouseWB

In Vitro Cell Dev Biol Anim

Mechanical stretch induces angiotensinogen expression through PARP1 activation in kidney proximal tubular cells.

Authors - Jeong Soon Lee
mouseWB

Int J Cardiol

ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in cardiomyocytes.

Authors - Baoshan Liu
mouseWB

Mol Pharmacol

Involvement of the spinal angiotensin II system in streptozotocin-induced diabetic neuropathic pain in mice.

Authors - Yoshiki Ogata
mouseIHC

Nutrients

Expression of Renin-Angiotensin System Components in the Taste Organ of Mice.

Authors - Noriatsu Shigemura
mouseWB

Sci Rep

Astragaloside IV protects against podocyte injury via SERCA2-dependent ER stress reduction and AMPKα-regulated autophagy induction in streptozotocin-induced diabetic nephropathy.

Authors - Hengjiang Guo