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AIRE Polyclonal antibody

AIRE Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22517-1-AP

Synonyms

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of L02 using 22517-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of U-937 using 22517-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of human spleen using 22517-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of mouse thymus using 22517-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using AIRE Polyclonal antibody (22517-1-AP)

    WB analysis of human plasma using 22517-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 22517-1-AP (AIRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thymus tissue using AIRE Polyclonal antibody (22517-1-AP)

    IHC staining of human thymus using 22517-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22517-1-AP (AIRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thymus tissue using AIRE Polyclonal antibody (22517-1-AP)

    IHC staining of human thymus using 22517-1-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 22517-1-AP (AIRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIRE Polyclonal antibody (22517-1-AP)

    IF Staining of HeLa using 22517-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 22517-1-AP (AIRE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, human plasma tissue, human spleen tissue, mouse thymus tissue, U-937 cells
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22517-1-AP targets AIRE in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIRE fusion protein Ag18249
Full Name autoimmune regulator
Calculated Molecular Weight 545 aa, 58 kDa
Observed Molecular Weight 58-69 kDa
GenBank Accession NumberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
RRIDAB_2879113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of the protein is not well defined, however it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described [1]. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Protocols

Product Specific Protocols
WB protocol for AIRE antibody 22517-1-APDownload protocol
IHC protocol for AIRE antibody 22517-1-APDownload protocol
IF protocol for AIRE antibody 22517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Immunol

Chd4 choreographs self-antigen expression for central immune tolerance.

Authors - Yoshihiko Tomofuji
View Article
mouseWB

Biochemistry (Mosc)

Defective Central Immune Tolerance Induced by High-Dose D-Galactose Resembles Aging.

Authors - H M Du
View Article
humanIF

Nat Commun

The immune landscape of human thymic epithelial tumors

Authors - Zhongwei Xin
View Article