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ALDH1A1-specific Polyclonal antibody

ALDH1A1-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22109-1-AP

Synonyms

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1



Tested Applications

Positive WB detected inhuman liver tissue, mouse liver tissue, human adrenal gland tissue, K-562 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22109-1-AP is ALDH1A1-specific Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A1-specific fusion protein Ag17358
Full Name aldehyde dehydrogenase 1 family, member A1
Calculated molecular weight 501 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC001505
Gene symbol ALDH1A1
Gene ID (NCBI) 216
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family. This antibody is specific to ALDH1A1.

Protocols

Product Specific Protocols
WB protocol for ALDH1A1-specific antibody 22109-1-APDownload protocol
IHC protocol for ALDH1A1-specific antibody 22109-1-APDownload protocol
IF protocol for ALDH1A1-specific antibody 22109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Renal cell carcinoma with synchronous ipsilateral urothelial carcinoma of the renal pelvis.

Authors - Qun Lu
humanWB

Theranostics

PMID: 28839463

Authors - Junlong Zhuang