Tested Applications
| Positive WB detected in | A549 cells, SK-BR-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IF/ICC detected in | C2C12 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86429-5-RR targets ALDH2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag7452 Product name: Source: e coli.-derived, PGEX-4T Tag: GST Sequence: Predict reactive species |
| Full Name | aldehyde dehydrogenase 2 family (mitochondrial) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002967 |
| Gene Symbol | ALDH2 |
| Gene ID (NCBI) | 217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH2 antibody 86429-5-RR | Download protocol |
| IF protocol for ALDH2 antibody 86429-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
