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AMBRA1 Antibody

AMBRA1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13762-1-AP

Synonyms

AMBRA1, autophagy/beclin 1 regulator 1, KIAA1736, WDR94



Tested Applications

Positive WB detected inMCF-7 cells, human brain tissue, HT-1080 cells, HepG2 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13762-1-AP targets AMBRA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMBRA1 fusion protein Ag4735
Full Name autophagy/beclin-1 regulator 1
Calculated molecular weight 1208 aa, 133 kDa
Observed molecular weight 130-135 kDa
GenBank accession numberBC045609
Gene symbol AMBRA1
Gene ID (NCBI) 55626
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.

Protocols

Product Specific Protocols
WB protocol for AMBRA1 antibody 13762-1-APDownload protocol
IHC protocol for AMBRA1 antibody 13762-1-APDownload protocol
IF protocol for AMBRA1 antibody 13762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Brain Pathol

AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.

Authors - Yasuo Miki
  • KD Validated