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ANGPTL7 Polyclonal antibody

ANGPTL7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10396-1-AP

Synonyms

angiopoietin like 7, Angiopoietin like factor, Angiopoietin like protein 7, Angiopoietin related protein 7, ANGPTL7, AngX, CDT6, RP4 647M16.2

Validation Data Gallery

  • WB analysis using 10396-1-AP

    WB analysis using 10396-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10396-1-AP (ANGPTL7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 10396-1-AP

    WB analysis of MCF-7 using 10396-1-AP

    WB result of ANGPTL7 antibody (10396-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANGPTL7 transfected MCF-7 cells.

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, COLO 320 cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

10396-1-AP targets ANGPTL7 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANGPTL7 fusion protein Ag0596
Full Name angiopoietin-like 7
Calculated molecular weight 40 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC001881
Gene symbol ANGPTL7
Gene ID (NCBI) 10218
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANGPTL7, also named as CDT6, is a member of the angiopoietin-like (ANGPTL) family of proteins, which are important regulators of Angiogenesis. It is a secreted 45-kDa glycoprotein that folds into a homotetramer structure linked by disulfide bonds. ANGPTL7 was originally discovered in the stromal layer of the corne, and moderately expressed in the human trabecular meshwork. The chromosomal location of the ANGPTL7 gene (1p36.22) is within the GLC3B locus (1p36.2-1p36.1) of primary congenital glaucoma, supporting ANGPTL7 as a candidate glaucoma gene. ANGPTL7 could have a pathogenic role in glaucoma, and may serve as a potential therapeutic target.

Protocols

Product Specific Protocols
WB protocol for ANGPTL7 antibody 10396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Genes Cells

Evidence for a role of angiopoietin-like 7 (ANGPTL7) in extracellular matrix formation of the human trabecular meshwork: implications for glaucoma.

Authors - Comes Núria N
View Article
humanWB

FASEB J

Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation.

Authors - Tong Xu
  • KD Validated
View Article