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  • KD/KO Validated

AP1M1 Polyclonal antibody

AP1M1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12112-1-AP

Synonyms

AP 1 complex subunit mu 1, AP mu chain family member mu1A, AP1M1, AP47, CLAPM2, CLTNM, MU 1A, Mu adaptin 1, Mu1A adaptin



Tested Applications

Positive WB detected inhuman brain tissue, Hacat cells
Positive IP detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

12112-1-AP targets AP1M1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP1M1 fusion protein Ag2757
Full Name adaptor-related protein complex 1, mu 1 subunit
Calculated molecular weight 423 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC017469
Gene symbol AP1M1
Gene ID (NCBI) 8907
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Protocols

Product Specific Protocols
WB protocol for AP1M1 antibody 12112-1-APDownload protocol
IP protocol for AP1M1 antibody 12112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virology

The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4(+) T cell apoptosis.

Authors - Rajesh Abraham Jacob
dog, mouseWB

PLoS One

Basolateral BMP signaling in polarized epithelial cells.

Authors - Saitoh Masao M
  • KD Validated
humanWB,IF

Traffic

AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.

Authors - Mathilde Bonnemaison
  • KD Validated
WB

J Biol Chem

Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.

Authors - Mathilde L Bonnemaison
  • KD Validated
humanWB

Nat Cell Biol

Plasma membrane contributes to the formation of pre-autophagosomal structures.

Authors - Ravikumar Brinda B
  • KD Validated