|Positive WB detected in||human brain tissue, Hacat cells|
|Positive IP detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
12112-1-AP targets AP1M1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AP1M1 fusion protein Ag2757|
|Full Name||adaptor-related protein complex 1, mu 1 subunit|
|Calculated molecular weight||423 aa, 49 kDa|
|Observed molecular weight||49 kDa|
|GenBank accession number||BC017469|
|Gene ID (NCBI)||8907|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
The interaction between HIV-1 Nef and adaptor protein-2 reduces Nef-mediated CD4(+) T cell apoptosis.
Basolateral BMP signaling in polarized epithelial cells.
AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.
J Biol Chem
Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.
Nat Cell Biol
Plasma membrane contributes to the formation of pre-autophagosomal structures.