Amphiregulin Monoclonal antibody

Amphiregulin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A1G9

Cat no : 66433-1-Ig

Synonyms

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF



Tested Applications

Positive WB detected inA549 cells, rat brain tissue, MCF-7 cells, pig brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66433-1-Ig targets Amphiregulin in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Amphiregulin fusion protein Ag8907
Full Name amphiregulin
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 50 kDa, 37 kDa
GenBank Accession NumberBC009799
Gene Symbol AREG
Gene ID (NCBI) 374
RRIDAB_2881803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Protocols

Product Specific Protocols
WB protocol for Amphiregulin antibody 66433-1-IgDownload protocol
IHC protocol for Amphiregulin antibody 66433-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Rep Med

Tumor-associated monocytes promote mesenchymal transformation through EGFR signaling in glioma

Authors - Yiyun Chen
humanWB

J Invest Dermatol

LPCAT1 promotes cutaneous squamous cell carcinoma via EGFR-mediated AKT/p38MAPK signaling pathways.

Authors - Yingjian Huang

Int J Biol Sci

CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17.

Authors - Jie Liu