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  • KD/KO Validated

RhoGDI Polyclonal antibody

RhoGDI Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10509-1-Ig

Synonyms

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1



Tested Applications

Positive WB detected inJurkat cells, A431 cells, mouse pancreas tissue, HL-60 cells, HeLa cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10509-1-Ig targets RhoGDI in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RhoGDI fusion protein Ag0791
Full Name Rho GDP dissociation inhibitor (GDI) alpha
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005851
Gene symbol ARHGDIA
Gene ID (NCBI) 396
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.

Protocols

Product Specific Protocols
WB protocol for RhoGDI antibody 10509-1-IgDownload protocol
IHC protocol for RhoGDI antibody 10509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

MiR-25 promotes hepatocellular carcinoma cell growth, migration and invasion by inhibiting RhoGDI1.

Authors - Congren Wang
  • KD Validated
humanWB

Tumour Biol

AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.

Authors - Zhaoyang Zeng
mouseWB

J Agric Food Chem

A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.

Authors - Cheng Liu
humanWB

PLoS One

Comparative expression profiling of distinct T cell subsets undergoing oxidative stress.

Authors - Lichtenfels Rudolf R
human, ratWB, IHC

PLoS One

Proteomic analysis of adrenocorticotropic hormone treatment of an infantile spasm model induced by N-methyl-D-aspartic acid and prenatal stress.

Authors - Wang Jing J
humanWB

J Exp Clin Cancer Res

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

Authors - Yu Lian