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RhoGDI Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 10509-1-Ig

Featured Product KD/KO validated

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Con: 90 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

RhoGDI Antibody WB Jurkat cells 10509-1-Ig

Jurkat cells were subjected to SDS PAGE followed by western blot with 10509-1-Ig(RhoGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

RhoGDI Antibody WB HEK-293 cells 10509-1-Ig

WB result of RhoGDI antibody (10509-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells

RhoGDI Antibody IHC human breast cancer tissue 10509-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 10x lens)

RhoGDI Antibody IHC human breast cancer tissue 10509-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer using 10509-1-Ig(RhoGDI antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
Jurkat cells, A431 cells, mouse pancreas tissue, HL-60 cells, HeLa cells, HEK-293 cells
Positive IHC detected in:
human breast cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Protein A purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
RhoGDI fusion protein Ag0791
Full name:
Rho GDP dissociation inhibitor (GDI) alpha
Calculated molecular weight:
28 kDa
Observed molecular weight:
28 kDa
GenBank accession number:
BC005851
Gene ID (NCBI):
396
Gene symbol
ARHGDIA
Synonyms
aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

Background

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.


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