ARHGDIB Antibody

ARHGDIB Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 16122-1-AP

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2



Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, Raji cells, rat spleen tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

16122-1-AP targets ARHGDIB in IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGDIB fusion protein Ag9078
Full Name Rho GDP dissociation inhibitor (GDI) beta
Calculated molecular weight 201aa,23 kDa
Observed molecular weight 27 kDa
GenBank accession number BC009200
Gene symbol ARHGDIB
Gene ID (NCBI) 397
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for ARHGDIB antibody 16122-1-APDownload protocol
IHC protocol for ARHGDIB antibody 16122-1-APDownload protocol
IP protocol for ARHGDIB antibody 16122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

DNA Cell Biol

Rho GDP Dissociation Inhibitor (GDI) Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma.

Authors - Yang Fang
humanWB

Proteomics

Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.

Authors - Fan Zhang
humanWB

Nat Immunol

Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.

Authors - Jing Geng