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ARHGDIB Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 16122-1-AP

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Con: 80 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
Application not tested? Please see our Guarantee


Images See all

ARHGDIB Antibody WB HL-60 cells 16122-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours

ARHGDIB Antibody IP Raji cells 16122-1-AP

IP Result of anti-ARHGDIB (IP:16122-1-AP, 4ug; Detection:16122-1-AP 1:1000) with Raji cells lysate 2000ug.

ARHGDIB Antibody IHC human stomach cancer tissue 16122-1-AP

Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP(ARHGDIB antibody) at dilution of 1:100 (under 10x lens)

ARHGDIB Antibody IHC human stomach cancer tissue 16122-1-AP

Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP(ARHGDIB antibody) at dilution of 1:100 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
HL-60 cells, Jurkat cells, K-562 cells, Raji cells, rat spleen tissue
Positive IP detected in:
Raji cells
Positive IHC detected in:
human stomach cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ARHGDIB fusion protein Ag9078
Full name:
Rho GDP dissociation inhibitor (GDI) beta
Calculated molecular weight:
201aa,23 kDa
Observed molecular weight:
27 kDa
GenBank accession number:
BC009200
Gene ID (NCBI):
397
Gene symbol
ARHGDIB
Synonyms
ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

Background

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.


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