ARHGDIB Antibody 16122-1-AP

Tested Applications

Applications:

ELISA, WB, IP, IHC
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Jurkat cells were subjected to SDS PAGE followed by western blot with 16122-1-AP(ARHGDIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IP Result of anti-ARHGDIB (IP:16122-1-AP, 4ug; Detection:16122-1-AP 1:1000) with Raji cells lysate 2000ug.

Immunohistochemical of paraffin-embedded human stomach cancer using 16122-1-AP(ARHGDIB antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human stomach cancer using 16122-1-AP(ARHGDIB antibody) at dilution of 1:100 (under 40x lens)

Species specificity:

human, mouse, rat

Positive WB detected in:

Jurkat cells, HL-60 cells, K-562 cells, Raji cells, rat spleen tissue

Positive IP detected in:

Raji cells

Positive IHC detected in:

human stomach cancer tissue

Recommended dilution:

WB : 1:500-1:2000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:20-1:200

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ARHGDIB fusion protein ag9078

Full name:

Rho GDP dissociation inhibitor (GDI) beta

Calculated molecular weight:

201aa,23 kDa

Observed molecular weight:

27 kDa

GenBank accession number:

BC009200

Gene ID (NCBI):

397

Gene symbol

ARHGDIB

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

Background

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Showing 1- [3] of [3] Publications

Author:		Yang Fang
Pubmed ID:		25180836
Journal:		DNA Cell Biol
Title:		Rho GDP Dissociation Inhibitor (GDI) Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma.
Application:		WB

Species:		human

Author:		Fan Zhang
Pubmed ID:		25411139
Journal:		Proteomics
Title:		Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.
Application:		WB

Species:		human

Author:		Jing Geng
Pubmed ID:		26414765
Journal:		Nat Immunol
Title:		Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.
Application:		WB

Species:		human

WB Figures

HL-60 cells were subjected to SDS PAGE followed by western blot with 16122-1-AP(ARHGDIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 16122-1-AP(ARHGDIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 16122-1-AP(ARHGDIB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Raji cells were subjected to SDS PAGE followed by western blot with 16122-1-AP(ARHGDIB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

rat spleen tissue were subjected to SDS PAGE followed by western blot with 16122-1-AP( ARHGDIB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human stomach cancer using 16122-1-AP(ARHGDIB antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human stomach cancer using 16122-1-AP(ARHGDIB antibody) at dilution of 1:100 (under 40x lens)

IP Figures

IP Result of anti-ARHGDIB (IP:16122-1-AP, 4ug; Detection:16122-1-AP 1:1000) with Raji cells lysate 2000ug.

ARHGDIB Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 16122-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [3] of [3] Publications

WB Figures

IHC Figures

IP Figures

ARHGDIB Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 16122-1-AP

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Tested Applications

Product Information

Immunogen Information

Background