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ARHGDIB Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66054-1-Ig |CloneNo.: 3E7B7

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Con: 151 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

ARHGDIB Antibody WB THP-1 cells 66054-1-Ig

Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours

ARHGDIB Antibody IHC human tonsillitis tissue 66054-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ARHGDIB Antibody IHC human tonsillitis tissue 66054-1-Ig

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
THP-1 cells, Raji cells, HL-60 cells, U-937 cells, Jurkat cells,
Positive IHC detected in:
human tonsillitis tissue, human cerebellum tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:10000-1:50000
IHC : 1:5000-1:10000
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ARHGDIB fusion protein Ag9091
Full name:
Rho GDP dissociation inhibitor (GDI) beta
Calculated molecular weight:
201aa,23 kDa
Observed molecular weight:
27 kDa
GenBank accession number:
BC009200
Gene ID (NCBI):
397
Gene symbol
ARHGDIB
Synonyms
ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

Background

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.


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