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ARHGDIB Monoclonal antibody

ARHGDIB Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

3E7B7

Cat no : 66054-1-Ig

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

Validation Data Gallery

  • WB analysis using 66054-1-Ig

    WB analysis using 66054-1-Ig

    Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66054-1-Ig

    IHC staining of human tonsillitis using 66054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66054-1-Ig

    IHC staining of human tonsillitis using 66054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inTHP-1 cells, Raji cells, HL-60 cells, U-937 cells, Jurkat cells,
Positive IHC detected inhuman tonsillitis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66054-1-Ig is ARHGDIB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARHGDIB fusion protein Ag9091
Full Name Rho GDP dissociation inhibitor (GDI) beta
Calculated molecular weight 201 aa, 23 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC009200
Gene symbol ARHGDIB
Gene ID (NCBI) 397
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for ARHGDIB antibody 66054-1-IgDownload protocol
IHC protocol for ARHGDIB antibody 66054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols