• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ARHGDIB Monoclonal antibody

ARHGDIB Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E7B7

Cat no : 66054-1-Ig

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ARHGDIB Monoclonal antibody (66054-1-Ig)

    WB analysis using 66054-1-Ig

    Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human tonsillitis using 66054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human tonsillitis using 66054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human cerebellum using 66054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IHC staining of human cerebellum using 66054-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IF Staining of human tonsillitis using 66054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ARHGDIB Monoclonal antibody (66054-1-Ig)

    IF Staining of human tonsillitis using 66054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inTHP-1 cells, Jurkat cells, U-937 cells, HL-60 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:50000
Immunohistochemistry (IHC)IHC : 1:5000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66054-1-Ig targets ARHGDIB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARHGDIB fusion protein Ag9091
Full Name Rho GDP dissociation inhibitor (GDI) beta
Calculated Molecular Weight 201 aa, 23 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC009200
Gene Symbol ARHGDIB
Gene ID (NCBI) 397
RRIDAB_11045657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Protocols

Product Specific Protocols
WB protocol for ARHGDIB antibody 66054-1-IgDownload protocol
IHC protocol for ARHGDIB antibody 66054-1-IgDownload protocol
IF protocol for ARHGDIB antibody 66054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hematology

Bioinformatic analysis of the expression profile and identification of RhoGDI2 as a biomarker in imatinib-resistant K562 cells

Authors - Yulin Yang
View Article