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Beta Arrestin 2 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 10171-1-AP

Featured Product KD/KO validated

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Con: 41 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Beta Arrestin 2 Antibody IF mouse Retina tissue 10171-1-AP

IF result of anti-ARRB2(10171-1-AP) in Mouse Retina by Dr. Seongjin Seo. IS(Inner segment) Stain.

Beta Arrestin 2 Antibody WB RAW264.7 10171-1-AP

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10171-1-AP(Beta Arrestin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Beta Arrestin 2 Antibody IHC human tonsil tissue 10171-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP( Beta Arrestin 2 antibody) at dilution of 1:200 (under 10x lens).

Beta Arrestin 2 Antibody IHC human tonsil tissue 10171-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 10171-1-AP( Beta Arrestin 2 antibody) at dilution of 1:200 (under 40x lens).

Beta Arrestin 2 Antibody IP RAW 264.7 cells 10171-1-AP

IP Result of anti-Beta Arrestin 2 (IP:10171-1-AP, 4ug; Detection:10171-1-AP 1:1000) with RAW 264.7 cells lysate 4000ug.

Species specificity:
human, mouse, Xenopus
Positive WB detected in:
RAW 264.7 cells, RAW264.7 cells
Positive IP detected in:
RAW 264.7 cells
Positive IHC detected in:
human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse Retina tissue
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Beta Arrestin 2 fusion protein Ag0227
Full name:
arrestin, beta 2
Calculated molecular weight:
46 kDa
Observed molecular weight:
50 kDa
GenBank accession number:
BC007427
Gene ID (NCBI):
409
Gene symbol
ARRB2
Synonyms
ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2

Background

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.


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