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- KD/KO Validated
Beta Arrestin 2 Polyclonal antibody
Beta Arrestin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10171-1-AP
|Positive WB detected in||RAW 264.7 cells, RAW264.7 cells|
|Positive IP detected in||RAW 264.7 cells|
|Positive IHC detected in||human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse Retina tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beta Arrestin 2 fusion protein Ag0227|
|Full Name||arrestin, beta 2|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC007427|
|Gene ID (NCBI)||409|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
β-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca(2+) Pathway in Xenopus Gastrulation.
Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the α7nAch receptor in SH-SY5Y cells.
Lactate enhances Arc/arg3.1 expression through hydroxycarboxylic acid receptor 1-β-arrestin2 pathway in astrocytes.
J Mol Med (Berl)
β-Arrestin-1 deficiency ameliorates renal interstitial fibrosis by blocking Wnt1/β-catenin signaling in mice.
Cell Death Dis
β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.
The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.