- Featured Product
- KD/KO Validated
Beta Arrestin 2 Polyclonal antibody
Beta Arrestin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10171-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells, RAW264.7 cells |
| Positive IP detected in | RAW 264.7 cells |
| Positive IHC detected in | human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse Retina tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 6 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Beta Arrestin 2 fusion protein Ag0227 |
| Full Name | arrestin, beta 2 |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC007427 |
| Gene symbol | ARRB2 |
| Gene ID (NCBI) | 409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
| IHC protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
| IP protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One β-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca(2+) Pathway in Xenopus Gastrulation. | ||
Sci Rep Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the α7nAch receptor in SH-SY5Y cells.
| ||
Neuropharmacology Lactate enhances Arc/arg3.1 expression through hydroxycarboxylic acid receptor 1-β-arrestin2 pathway in astrocytes.
| ||
J Mol Med (Berl) β-Arrestin-1 deficiency ameliorates renal interstitial fibrosis by blocking Wnt1/β-catenin signaling in mice. | ||
Cell Death Dis β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.
| ||
Development The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning. |
