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  • KD/KO Validated

Beta Arrestin 2 Polyclonal antibody

Beta Arrestin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10171-1-AP

Synonyms

ARB2, ARR2, ARRB2, Arrestin beta 2, arrestin, beta 2, BARR2, Beta arrestin 2



Tested Applications

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse Retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10171-1-AP targets Beta Arrestin 2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, Xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Arrestin 2 fusion protein Ag0227
Full Name arrestin, beta 2
Calculated molecular weight 46 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC007427
Gene symbol ARRB2
Gene ID (NCBI) 409
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

Protocols

Product Specific Protocols
WB protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IHC protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IP protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
XenopusWB

PLoS One

β-Arrestin Interacts with the Beta/Gamma Subunits of Trimeric G-Proteins and Dishevelled in the Wnt/Ca(2+) Pathway in Xenopus Gastrulation.

Authors - Katharina Seitz
humanWB

Sci Rep

Arrestins contribute to amyloid beta-induced cell death via modulation of autophagy and the α7nAch receptor in SH-SY5Y cells.

Authors - Yi-Qing Liu
  • KD Validated
mouseWB

Neuropharmacology

Lactate enhances Arc/arg3.1 expression through hydroxycarboxylic acid receptor 1-β-arrestin2 pathway in astrocytes.

Authors - Kaiyang Ma
  • KO Validated
human, mouseWB,IHC

J Mol Med (Berl)

β-Arrestin-1 deficiency ameliorates renal interstitial fibrosis by blocking Wnt1/β-catenin signaling in mice.

Authors - Huiyan Xu
mouseWB,IHC,IF

Cell Death Dis

β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.

Authors - J Liu
  • KD Validated
humanWB

Development

The phosphatase Pgam5 antagonizes Wnt/β-Catenin signaling in embryonic anterior-posterior axis patterning.

Authors - Verena Rauschenberger