ASC/TMS1 Monoclonal antibody
ASC/TMS1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat and More (1)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2G12E6
Cat no : 67494-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HL-60 cells, human placenta tissue, rat spleen tissue, THP-1 cells, U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 20 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
67494-1-Ig targets ASC/TMS1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ASC/TMS1 fusion protein Ag28424 |
| Full Name | PYD and CARD domain containing |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC004470 |
| Gene Symbol | PYCARD |
| Gene ID (NCBI) | 29108 |
| RRID | AB_2882718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASC/TMS1 antibody 67494-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Oxid Med Cell Longev PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway. | ||
J Neuroinflammation Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice. | ||
J Virol Pseudorabies Virus Infection Activates the TLR-NF-κB Axis and AIM2 Inflammasome To Enhance Inflammatory Responses in Mice | ||
J Ethnopharmacol Xiaoqinglong decoction improves allergic rhinitis by inhibiting NLRP3-mediated pyroptosis in BALB/C mice |
