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ATG5 Monoclonal antibody

ATG5 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3G6E6

Cat no : 60061-1-Ig

Synonyms

APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5



Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60061-1-Ig targets ATG5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen ATG5 fusion protein Ag0214
Full Name ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight 32 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002699
Gene symbol ATG5
Gene ID (NCBI) 9474
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.

Protocols

Product Specific Protocols
WB protocol for ATG5 antibody 60061-1-IgDownload protocol
IHC protocol for ATG5 antibody 60061-1-IgDownload protocol
IF protocol for ATG5 antibody 60061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Autophagy

The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.

Authors - Andreas Struchtrup
mouseWB

Autophagy

Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.

Authors - Qian Huang