|Positive WB detected in||K-562 cells, HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60061-1-Ig targets ATG5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgM|
|Immunogen||ATG5 fusion protein Ag0214|
|Full Name||ATG5 autophagy related 5 homolog (S. cerevisiae)|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC002699|
|Gene ID (NCBI)||9474|
|Purification Method||Caprylic acid/ammonium sulfate precipitation|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.
Mol Med Rep
Autophagy is essential for flavopiridol‑induced cytotoxicity against MCF‑7 breast cancer cells.
The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.
Autophagy core protein ATG5 is required for elongating spermatid development, sperm individualization and normal fertility in male mice.