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BID Polyclonal antibody

BID Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 10988-1-AP

Synonyms

BID, FP497, p11 BID, p22 BID



Tested Applications

Positive WB detected inA431 cells, HeLa cells, human brain tissue, Tunicamycin treated HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 32 publications below

Product Information

10988-1-AP targets BID in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BID fusion protein Ag1445
Full Name BH3 interacting domain death agonist
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009197
Gene symbol BID
Gene ID (NCBI) 637
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)

Protocols

Product Specific Protocols
WB protocol for BID antibody 10988-1-APDownload protocol
IP protocol for BID antibody 10988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Immunol

Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1β During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation.

Authors - Yi Liao
humanWB

Cancers (Basel)

Cinnamaldehyde Enhances Antimelanoma Activity through Covalently Binding ENO1 and Exhibits a Promoting Effect with Dacarbazine.

Authors - Weiyi Zhang
humanWB

Cell Death Dis

LINC00266-1/miR-548c-3p/SMAD2 feedback loop stimulates the development of osteosarcoma.

Authors - Shengnai Zheng
humanWB

Cell Death Dis

BioID-based proteomic analysis of the Bid interactome identifies novel proteins involved in cell-cycle-dependent apoptotic priming.

Authors - Robert Pedley
WB

Toxicol Mech Methods

ML-7 amplifies the quinocetone-induced cell death through akt and MAPK-mediated apoptosis on HepG2 cell line.

Authors - Yan Zhou
humanWB

Int J Clin Exp Med

mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma.

Authors - Lijing Zhao