|Positive WB detected in||A431 cells, HeLa cells, human brain tissue, Tunicamycin treated HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10988-1-AP targets BID in WB, IP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BID fusion protein Ag1445|
|Full Name||BH3 interacting domain death agonist|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC009197|
|Gene ID (NCBI)||637|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)
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