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BID Polyclonal antibody

BID Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10988-1-AP

Synonyms

BID, FP497, p11 BID, p22 BID



Tested Applications

Positive WB detected inA431 cells, human brain tissue, Tunicamycin treated HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10988-1-AP targets BID in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BID fusion protein Ag1445
Full Name BH3 interacting domain death agonist
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
RRIDAB_2065624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)

Protocols

Product Specific Protocols
WB protocol for BID antibody 10988-1-APDownload protocol
IP protocol for BID antibody 10988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Bioact Mater

A ROS-responsive, self-immolative and self-reporting hydrogen sulfide donor with multiple biological activities for the treatment of myocardial infarction.

Authors - Mengyun Yao
mouseWB

Environ Pollut

Silica nanoparticles inducing the apoptosis via microRNA-450b-3p targeting MTCH2 in mice and spermatocyte cell.

Authors - Guiqing Zhou
ratWB

Cell Mol Life Sci

Clostridioides difficile toxin B alone and with pro-inflammatory cytokines induces apoptosis in enteric glial cells by activating three different signalling pathways mediated by caspases, calpains and cathepsin B.

Authors - Katia Fettucciari
humanWB

Int J Cancer

Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma.

Authors - Liu Chaoyang C
humanWB

Free Radic Biol Med

Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma.

Authors - Gong Ke K
ratWB

Stem Cell Res Ther

Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.

Authors - Chaoling Yao