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BPNT1 Polyclonal antibody

BPNT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16461-1-AP

Synonyms

Bisphosphate 3 nucleotidase 1, BPNT1, PAP inositol 1,4 phosphatase, PIP

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of HEK-293 using 16461-1-AP

    HEK-293 and Hela cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of HeLa using 16461-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of human kidney using 16461-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BPNT1 Polyclonal antibody (16461-1-AP)

    WB analysis of HEK-293 using 16461-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16461-1-AP (BPNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using BPNT1 Polyclonal antibody (16461-1-AP)

    IHC staining of human liver using 16461-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using BPNT1 Polyclonal antibody (16461-1-AP)

    IHC staining of human liver using 16461-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16461-1-AP (BPNT1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BPNT1 Polyclonal antibody (16461-1-AP)

    IF Staining of HeLa using 16461-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16461-1-AP (BPNT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human kidney tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16461-1-AP targets BPNT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BPNT1 fusion protein Ag9523
Full Name 3'(2'), 5'-bisphosphate nucleotidase 1
Calculated Molecular Weight 325 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC017801
Gene Symbol BPNT1
Gene ID (NCBI) 10380
RRIDAB_2067193
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BPNT1 antibody 16461-1-APDownload protocol
IHC protocol for BPNT1 antibody 16461-1-APDownload protocol
IF protocol for BPNT1 antibody 16461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

PLoS One

Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea

Authors - Jing Liu
View Article