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BRIP1(N-terminal) Polyclonal antibody

BRIP1(N-terminal) Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24436-1-AP

Synonyms

BACH1, BRIP1, FANCJ, OF

Validation Data Gallery

  • WB analysis of HeLa using 24436-1-AP

    WB analysis of HeLa using 24436-1-AP

    WB result of BRIP1(N-terminal) antibody (24436-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BRIP1(N-terminal) transfected HeLa cells.

  • IF Staining of HeLa using 24436-1-AP

    IF Staining of HeLa using 24436-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 24436-1-AP (BRIP1(N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse testis using 24436-1-AP

    WB analysis of mouse testis using 24436-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24436-1-AP (BRIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24436-1-AP is BRIP1(N-terminal) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRIP1(N-terminal) fusion protein Ag19907
Full Name BRCA1 interacting protein C-terminal helicase 1
Calculated molecular weight 1249 aa, 141 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC101472
Gene symbol BRIP1
Gene ID (NCBI) 83990
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRIP1 also named as BACH1, FANCJ or ATP dependent RNA helicase BRIP1 is a 1249 amino acid protein, which contains one helicase ATP-binding domain and belongs to the DEAD box heilicase family, DEAH subfamily. BRIP1 contains nuclear localization signal and is ubiquitously expressed with highest levels in testis, an expression pattern similar to that of BRCA1. BRIP1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.

Protocols

Product Specific Protocols
WB protocol for BRIP1(N-terminal) antibody 24436-1-APDownload protocol
IF protocol for BRIP1(N-terminal) antibody 24436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols