BTG1 Polyclonal antibody

BTG1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14879-1-AP

Synonyms

BTG1, Protein BTG1



Tested Applications

Positive IHC detected inhuman thyroid cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14879-1-AP targets BTG1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity HUMAN, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTG1 fusion protein Ag6651
Full Name B-cell translocation gene 1, anti-proliferative
Calculated molecular weight 19 kDa
GenBank accession numberBC064953
Gene symbol BTG1
Gene ID (NCBI) 694
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTG1 is a member of the TOB/BTG family of proteins known to inhibit cell proliferation and negatively regulate the cell cycle (PMID: 25017022). It is strongly expressed in quiescent cells (maximal in the G0/G1 phase) and down-regulated as the cells enter the growth cycle (PMID: 1373383). BTG1 protein is localized in cytoplasm and nucleus, and can interact with CAF1 and PRMT1 (PMID: 1113675, 9712883, 26622543). The aberration of BTG1 expression is associated with B-cell lymphocytic leukemia, and may serve as a diagnostic indicator and prognostic marker of thyroid carcinoma (PMID: 2069907, 25017022).

Protocols

Product Specific Protocols
IHC protocol for BTG1 antibody 14879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

miR-27a-3p regulates proliferation and apoptosis of colon cancer cells by potentially targeting BTG1.

Authors - Chang Su

Int J Mol Med

B cell translocation gene 1 reduces the biological outcome of kidney cancer through induction of cell proliferation, cell cycle arrest, cell apoptosis and cell metastasis.

Authors - Guogui Sun
human,mouseWB,IF

J Cell Physiol

Long noncoding RNA DGCR5 suppresses gastric cancer progression by acting as a competing endogenous RNA of PTEN and BTG1.

Authors - Ying Xu

Int J Mol Sci

BTG1 expression correlates with the pathogenesis and progression of ovarian carcinomas.

Authors - Yang Zhao
HUMANWB,IHC

Oncotarget

BTG1 expression correlates with pathogenesis, aggressive behaviors and prognosis of gastric cancer: a potential target for gene therapy.

Authors - Hua-chuan Zheng