|Positive WB detected in||NIH/3T3 cells, RAW 264.7 cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human testis tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The 26967-1-AP immunogen is Fusion Protein Bcl-XL protein expressed in E. coli.
|Cited Species||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bcl-XL fusion protein Ag25604|
|Full Name||BCL2-like 1|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC019307|
|Gene ID (NCBI)||598|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).
|Product Specific Protocols|
|WB protocol for Bcl-XL antibody 26967-1-AP||Download protocol|
|IHC protocol for Bcl-XL antibody 26967-1-AP||Download protocol|
|IF protocol for Bcl-XL antibody 26967-1-AP||Download protocol|
|IP protocol for Bcl-XL antibody 26967-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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