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WB analysis of NIH/3T3 using 26967-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 26967-1-AP
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of NIH/3T3 using 26967-1-AP
IP result of anti-Bcl-XL (IP:26967-1-AP, 4ug; Detection:26967-1-AP 1:1000) with NIH/3T3 cells lysate 2000 ug.
IHC staining of human testis using 26967-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 26967-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human lung cancer using 26967-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 26967-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL/S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 26967-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Bcl-XL antibody (26967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of Jurkat using 26967-1-AP
1x10^6 200 Jurkat cells were intracellularly stained with 0.4 ug Bcl-XL Polyclonal antibody (26967-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

BCL2L1 Polyclonal antibody

BCL2L1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 26967-1-AP

Publications(77)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Bcl-xL, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1, Bcl-2-like protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (77)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, RAW 264.7 cells
Positive IP detected in	NIH/3T3 cells
Positive IHC detected in	human testis tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	200 Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 75 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

26967-1-AP targets BCL2L1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25604 Product name: Recombinant human Bcl-xL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-128 aa of BC019307 Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVN Predict reactive species
Full Name	BCL2-like 1
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC019307
Gene Symbol	BCL2L1
Gene ID (NCBI)	598
RRID	AB_2880702
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q07817
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Protocols

Product Specific Protocols
IF protocol for BCL2L1 antibody 26967-1-AP	Download protocol
IHC protocol for BCL2L1 antibody 26967-1-AP	Download protocol
IP protocol for BCL2L1 antibody 26967-1-AP	Download protocol
WB protocol for BCL2L1 antibody 26967-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy Authors - Lihao Jin View Article
rat	WB	Microbiome The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids. Authors - Weiping Xiao View Article
human	WB	Clin Cancer Res Long noncoding RNA LINC01234 functions as a competing endogenous RNA to regulate CBFB expression by sponging miR-204-5p in gastric cancer. Authors - Xin Chen View Article
human	WB	Clin Cancer Res Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-mutated Acute Myeloid Leukemia. Authors - Jun Ma View Article
human	WB	Cell Rep Developmental potency of human ES cell-derived mesenchymal stem cells revealed in mouse embryos following blastocyst injection Authors - Borong Huang View Article
mouse	WB	Int J Biol Macromol CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression Authors - Zhongqiu Zhou View Article