Bcl-XL Antibody

Bcl-XL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 26967-1-AP

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-xL



Tested Applications

Positive WB detected inNIH/3T3 cells, RAW 264.7 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman testis tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26967-1-AP immunogen is Fusion Protein Bcl-XL protein expressed in E. coli.

Reactivity human, mouse
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag25604
Full Name BCL2-like 1
Calculated molecular weight 26 kDa
Observed molecular weight 30 kDa
GenBank accession number BC019307
Gene symbol BCL2L1
Gene ID (NCBI) 598
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Protocols

Product Specific Protocols
WB protocol for Bcl-XL antibody 26967-1-APDownload protocol
IHC protocol for Bcl-XL antibody 26967-1-APDownload protocol
IF protocol for Bcl-XL antibody 26967-1-APDownload protocol
IP protocol for Bcl-XL antibody 26967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Metab Brain Dis

Protective effects of ethyl gallate on H2O2-induced mitochondrial dysfunction in PC12 cells.

Authors - Lan Chen
humanWB

Cancer Biol Ther

Deletion of MBD2 inhibits proliferation of chronic myeloid leukaemia blast phase cells.

Authors - Ling Cheng
humanWB

Biochim Biophys Acta

Nogo-B (Reticulon-4B) functions as a negative regulator of the apoptotic pathway through the interaction with c-FLIP in colorectal cancer cells.

Authors - Nao Kawaguchi
humanWB

Med Sci Monit

Mechanisms of IFNalpha-1a-Induced Apoptosis in a Laryngeal Cancer Cell Line.

Authors - Xiao-Lei Xin
humanWB

Oncol Lett

LINC00365-SCGB2A1 axis inhibits the viability of breast cancer through targeting NF-κB signaling.

Authors - Lichao Zhang
humanWB

FEBS Open Bio

Loss of scinderin decreased expression of epidermal growth factor receptor and promoted apoptosis of castration-resistant prostate cancer cells.

Authors - Xiaofeng Lai