CoraLite® Plus 647-conjugated Bcl-XL Polyclonal antibody
Bcl-XL Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-26967
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-26967 targets Bcl-XL in IF, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Bcl-XL fusion protein Ag25604 |
| Full Name | BCL2-like 1 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC019307 |
| Gene Symbol | BCL2L1 |
| Gene ID (NCBI) | 598 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 Bcl-XL antibody CL647-26967 | Download protocol |
| FC protocol for CL Plus 647 Bcl-XL antibody CL647-26967 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Alexia (Verified Customer) (07-25-2023) | Murine thymocytes were stained for 90 minutes at 4°C in the dark. The stain is extremely convincing compared to FMO.
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